The analysis of the de-silencing mechanism in E. coli

大肠杆菌去沉默机制分析

• 批准号: 23580114
• 负责人: OSHIMA Taku
• 金额: $ 3.24万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580114/
• 关键词: 微生物遺伝・育種; 転写制御; 核様体; 大腸菌; サイレンシング; 微生物; H-NS

The transcriptome analysis with the deletion mutants of hha, ydgT, stpA and hns genes indicated that Hha and YdgT are the proteins which assist H-NS to repress the expression over 100 genes. In addition, lux and lacZ reporter analysis to elucidate the mechanisms of the transcriptional regulations in ybdO and ler promoters indicated that the wide areas in up and downstream of the ybdO promoter is essential for the transcriptional repression of ybdO which depends on H-NS and the derepression of ler promoter with Pch protein observed when the repression of ler genes was cooperatively regulated by H-NS and Hha.

使用HHA，YDGT，STPA和HNS基因的缺失突变体的转录组分析表明，HHA和YDGT是蛋白质，可帮助H-NS抑制100个基因上的表达。 In addition, lux and lacZ reporter analysis to elucidate the mechanisms of the transcriptional regulations in ybdO and ler promoters indicated that the wide areas in up and downstream of the ybdO promoter is essential for the transcriptional repression of ybdO which depends on H-NS and the derepression of ler promoter with Pch protein observed when the repression of ler genes was cooperatively regulated by H-NS and Hha.

项目成果

バクテリアのサイレンシングの多様性と共通性

细菌沉默的多样性和共性

• 发表时间: 2012
• 作者: Wada;H.;Iwasaki;M.;and Kawakami;K.;Ryo Ikehata;F.-N. Meng and T. Oakuma;四方哲也;Ken'ichi Ohshika;T.Sakasai;大島 拓
• 通讯作者: 大島 拓

細菌における外来遺伝子発現抑性機構

抑制细菌外源基因表达的机制

• 发表时间: 2014
• 作者: Ogino;K.;Low;S.E.;Yamada;K.;Saint-Amant;L.;Zhou;W.;Muto;A.;Asakawa;K.;Nakai;J.;Kawakami;K.;Kuwada;J.Y.;and Hirata;H.;大鹿健一;E.Kin;舟木直久;大島 拓
• 通讯作者: 大島 拓

ゲノムワイドな解析手法により明らかになった核様体タンパク質の機能

全基因组分析方法揭示核蛋白的功能

• 发表时间: 2013
• 作者: Kawakami;K.;大島 拓
• 通讯作者: 大島 拓

Functions of the Hha and YdgT proteins in transcriptional silencing by the nucleoid proteins, H-NS and StpA, in Escherichia coli.

• DOI: 10.1093/dnares/dst008
• 发表时间: 2013-06
• 期刊: DNA research : an international journal for rapid publication of reports on genes and genomes
• 作者: Ueda T;Takahashi H;Uyar E;Ishikawa S;Ogasawara N;Oshima T
• 通讯作者: Oshima T

細菌における外来遺伝子発現抑制機構

抑制细菌外源基因表达的机制

• 发表时间: 2014
• 作者: Tsukahara;T.;Iwase;N.;Kawakami;K.;Iwasaki;M.;Yamamoto;C.;Ohmine;K.;Uchibori;R.;Teruya;T.;Ido;H.;Saga;Y.;Urabe;M.;Mizukami;H.;Kume;A.;Nakamura;M.;Brentjens;R.;and Ozawa;K.;大島 拓
• 通讯作者: 大島 拓