Development a novel method to analyze blood vessel occurrence in metanephroi and its network construction

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How blood vessel originated in metanephros is a question discussed for a long time. Angiogenesis and vasculogenesis are two main hypotheses. The former is related to blood vessel invasion, and the latter differentiation of progenitors from mesenchymal cells. However, which one is prerequisite to the very early stage has not been clarified. We developed a method to elucidate temporal angiogenesis originated from dorsal aorta and established a method to constitute the blood vessel network by applying the angiogenesis.Using resin combined with ink-template method to inject into umbilical cord, we succeeded in the identification of occurrence time of angiogenesis. In vivo transplantation subsequent to in vitro culture, we succeeded in generation of a full blood vessel network in kidney. These techniques are useful to both kidney and other organs.