Integrated functionnal analyes of insulin-like peptides which regutate diapause and lifespan in Caenor habditis elegans

点击翻译按钮获取中文摘要

Insulin/IGF-1 signaling is known to regulate larval diapause and lifespan of the nematode Caenorhabditis elegans. So far, 40 of insulin-like genes have been identified in C. elegans. So far, 40 of insulin-like genes have been identified in C. elegans. In order to identify an insulin-like molecule which physiological function is unknown as well as to elucidate how insulin-like molecules co-operatively regulate diapause and lifespan, we performed three experiments as follows. 1, a functional analysis in "molecular level" using molecular modeling; 2, a functional analysis in "cellular level" using a primary culture; 3, a functional analysis in "organism level" using molecular biological technics. Moreover, we proposed a model for regulatory mechanism of expression of an insulin-like gene.