Cellulose hydorogel for three-dimensional scaffold of cell culture

用于细胞培养三维支架的纤维素水凝胶

• 批准号: 23580226
• 负责人: WADA Masahisa
• 金额: $ 3.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580226/
• 关键词: セルロース; ヒドロゲル; 孔径制御; 細胞培養; 足場材料; 圧縮試験; 細胞足場材料

Cellulose was completely dissolved in the 60 wt% lithium bromide aqueous solution in the temperature 120 degrees. Then the cellulose solution was poured into glass container containing large excess amount of salt (NaCl) particles that was heated at 120 degrees in advance. The cellulose was regenerated from the solution by cooling down to room temperature and removing the slats by washing with water. The cellulose gel thus obtained has a uniform and large pore size more than 0.1 mm in diameter. The particle size of salts used in the process leads to the control of morphology and mechanical properties of the gel. The gels were seeded with rabbit chondrocyte and cultured in vitro for three weeks, and proliferation of chondrocyte and its differentiation to cartilagines were observed. In consequence, the cellulose gel has a possibility using as a three-dimensional scaffold for cell culture of tissue engineering.

将纤维素完全溶解在温度120度的60 wt％溴化物水溶液中。然后将纤维素溶液倒入玻璃容器中，其中包含大量过量的盐（NaCl）颗粒，这些颗粒会提前在120度加热。通过冷却到室温并用水洗涤板条，从溶液中再生。因此获得的纤维素凝胶的直径大于0.1 mm。该过程中使用的盐的粒径导致控制凝胶的形态和机械性能。将凝胶与兔软骨细胞接种，并在体外培养三周，并观察到软骨细胞的增殖，并观察到其与软骨的分化。因此，纤维素凝胶有可能用作组织工程细胞培养的三维支架。