Study on the olfactory imprinting-related brain regions in anadromous salmon: Neuroanatomical analysis for the primary olfactory projected regions in salmon brain.

为了弄清淡水型鲑鱼(Oncorhynchus spp.)的印记相关脑区，我们利用DII荧光示踪剂的神经解剖学技术对鲑鱼脑内初级嗅觉投射区域进行了可视化。为了区分构成每个初级嗅觉投射的嗅觉受体细胞的类型，我们用免疫组织化学方法分析了嗅觉标记蛋白和谷胱甘肽S转移酶pi作为嗅觉受体细胞标记物。该方法能够区分纤毛和微绒毛嗅觉感受器细胞。此外，利用激光共聚焦扫描显微镜对G蛋白α亚单位olf(Golf)在嗅觉相关的第二信使级联中的定位进行了免疫组织化学分析，并首次在鲑鱼嗅觉系统中检测到Golf的表达。

In order to clarify the imprinting-related brain regions in anadromous salmon (Oncorhynchus spp.), we visualized the primary olfactory projected regions in salmon brain by neuroanatomical techniques using DiI fluorescent tracer. In order to distinguish the type of the olfactory receptor cells which constitutes each primary olfactory projection, we analyzed immunohistochemically as olfactory receptor cell markers taking notice of the olfactory marker protein (OMP) and glutathione S-transferase pi (GST). This method was able to distinguish ciliated and microvillus olfactory receptor cells. In addition, the localization of G protein alpha subunit olf (Golf) in regard of a olfaction-related second messenger cascade was immunohistochemically analyzed using the confocal laser scanning microscope, and had expression of Golf identified for the first time in the salmon olfactory system.