A study on ASP toxin production and dynamics in toxin producing organisms

产毒生物中 ASP 毒素的产生和动态研究

• 批准号: 23580285
• 负责人: KOTAKI Yuichi
• 金额: $ 3.49万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580285/
• 关键词: 記憶喪失性貝毒; ドウモイ酸; イソドウモイ酸; Pseudo-nitzschia; Nitzschia; navis-varingica; ハナヤナギ; 黒潮; 細菌; 分布特性; 珪藻; multiseries

Distribution, ASP toxin production characteristics and the dynamics of these toxins were surveyed for the toxin producing diatom, Pseudo-nitszchia multiseries, Nitzschia navis-varingica and red alga Chondria armata. It was hypothesized that N. navis-varingica is a tropical species and expanded its distribution to eastern Japan along the warm water current "Kuroshio" which flows from the east coast of the Philippines to Japan. The characteristics of growth and the geographical comparison in the toxin profiles and genetic markers in N. navis-varingica strains along the "Kuroshio" supported the above hypothesis. N. navis-varingica shows the toxin profiles composed of domoic acid and isodomoic acids A and B. Factors controlling the toxin profiles were searched, resulting in finding the important roles of genetic difference and environmental bacteria. Bacterial effect on the toxin production seemed to be the most obvious in P. multiseries culture than in other organisms cultures.

对产毒硅藻、多系拟菱形藻、海军菱形藻和红藻Chondria armata的分布、ASP产毒特征及其动态进行了调查。据推测，N. navis-varingica 是热带物种，沿着从菲律宾东海岸流向日本的暖水流“黑潮”将其分布扩展到日本东部。沿着“黑潮”的N. navis-varingica菌株的生长特征以及毒素谱和遗传标记的地理比较支持了上述假设。 N. navis-varingica 显示由软骨藻酸和异软骨藻酸 A 和 B 组成的毒素谱。对控制毒素谱的因素进行了搜索，结果发现遗传差异和环境细菌的重要作用。与其他生物体培养物相比，多系假单胞菌培养物中细菌对毒素产生的影响似乎最为明显。

项目成果

記憶喪失性貝毒生産底生珪藻Nitzschia navis-varingicaの分布および毒生産特性-6

遗忘性贝类产毒底栖硅藻 Nitzschia navis-varingica-6 分布及产毒特性

• 发表时间: 2012
• 作者: R. Tatusno;K. Yamaguchi;T. Takatani and O. Arakawa;小瀧裕一・木田龍介・影山一成・原口浩一・Lundholm Nina
• 通讯作者: 小瀧裕一・木田龍介・影山一成・原口浩一・Lundholm Nina

震災後の記憶喪失性貝毒生産珪藻出現調査

震后遗忘性贝类产毒硅藻出现情况调查

• 作者: S. Niina;T. Araki;Y. Yamanaka;J. Wang;R. Tatsuno;O. Arakawa and T. Takatani;Hong PARK;小瀧裕一・吉田健一・ 境野智・品田祥太
• 通讯作者: 小瀧裕一・吉田健一・ 境野智・品田祥太

Factors affecting the ASP toxin production of a benthic diatom Nitzschia navis-varingica

影响底栖硅藻 Nitzschia navis-varingica ASP 毒素产生的因素

• 发表时间: 2012
• 作者: Yuichi Kotaki;Marc Lawrence J. Romero;Nina Lundholm;Juan R. Relox;Atsushi Kobiyama;Koichi Haraguchi
• 通讯作者: Koichi Haraguchi

記憶喪失性貝毒生産珪藻Nitzschia navis-varingicaの分布および毒生産特性ー５

遗忘性贝类产毒硅藻 Nitzschia navis-varingica - 5 的分布及产毒特性

• 发表时间: 2011
• 作者: R. Tatsuno;M. Shikina;Y. Shirai;T. Takatani;and O. Arakawa;朴紅;小瀧裕一・冨山崇・澤口敏弥・Lundholm Nina
• 通讯作者: 小瀧裕一・冨山崇・澤口敏弥・Lundholm Nina

Two new ASP toxin production types in strains of Nitzschia navis-varingica from the Philippines

菲律宾菱形藻菌株中两种新的 ASP 毒素产生类型

• 发表时间: 2012
• 期刊: Coastal Marine Science
• 作者: Romero;M. L. J.;Kotaki;Y.;Relox;J. Jr.;Kundholm;N.;Takata;Y.;Kodama;M. and Fukuyo Y.
• 通讯作者: M. and Fukuyo Y.