A development of near infrared imaging spectroscopy with heavy water to visualise water movement in plants

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Because the chemical characteristics is similar to water, deuterium oxide (D2O) well up-taken in plants can be a good tracer to study plant water relations. At a specific near infrared waveband, near-infrared is absorbed not by D2O but by water. Therefore, a combination of near-infrared imaging and D2O tracer provides a method observing water movement in plants. In this study, we established macroscopic and microscopic visualization methods to investigate water movement in plants. We also developed an user interface for this novel method.