Development of techniques for establishing the fully human antibody high producing human cell lines

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To produce fully human antibodies, cell fusion were examined using human cells. As a result, it was found that the cell fusion using the T lymphoid fusion partner such as A4H12 could generate universally human hybridomas that formed cell aggregation and increased their antibody production by retinoic acid. For the cell fusion, the quality of fetal bovine serum was the most important factor.In addition, the production of recombinant fully human antibodies was also challenged. The introduction of the fructose transporter GLUT5 and antibody co-expression system into the human cell line SC-01MFP made it possible to produce them in the fructose-based medium up to about two-fold of the conventional glucose-based medium.