Ion channel coupling and membrane plasticity in nucleus and mitochondria

将腔内Ca2+浓度固定在超过10微摩尔浓度的核/内质网制剂（核膜：NE）中，并将其置于膜片钳记录技术的全NE配置中，我观察到膜电容的增加，即膜面积的测量。在原位膜片钳条件下记录的膜电容在显微镜下波动。结果表明，NE膜系统表现出反映时间膜囊泡和塌陷的特征性波动，这一过程受腔内Ca2+浓度控制。NE膜具有丰富的离子通道和水通道。这些活性膜可能有助于维持NE的组装、管腔和核膜的结构。此外，我用激光共聚焦显微镜观察到，在NE制备过程中，有一层厚膜堆积，在一个小点上堆叠成层，假设与核孔对应。

Fixing the lumen Ca2+-concentration at over 10 micro molar concentration in a nuclear/endoplasmic reticulum preparation (nuclear envelope: NE), which is subjected into whole-NE configuration of the patch-clamp recording techniques, I observed increases of membrane electrical capacitance, the measure of the membrane area. The membrane capacitance, recorded under in situ patch-clamp condition, fluctuated microscopically. The result suggests that the NE membrane system shows a characteristic undulation reflecting temporal membrane vesiculation and collapse, and the process is under control of the lumen Ca2+ concentration. The NE membrane is rich in ion- and water- channels. Those active membrane may component contribute to maintain NE assembly, the structure of the lumen and the nuclear membranes. Further, I observed with laser confocal microscopy in the NE preparation, a thick membrane accumulation, stacking into layers at a small spot, assumingly corresponding to the nucleic pore.