High time resolution FRET analysis of the activation of G protein

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G protein coupled receptors (GPCRs) are the important signaling molecules which are activated upon the binding with the specific ligand and trigger the downstream signaling through activating G protein. These initial processes of GPCR signaling, the activation of receptor and the following G protein binding, were analyzed by using a fluorescence resonance energy transfer (FRET) technique. With the high time resolution FRET analysis, we found that binding of G protein stabilizes the active conformations of GPCRs and slows the deactivation of the activated receptor. Further FRET studies revealed that the effects of G protein binding differ depending on the type of the coupled receptors.