Studies on the mechanisms of insecticide resistance in chikungunya mosquito vectors

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In order to construct more appropriate strategies for controlling mosquito vectors to reduce the risk of Chikungunya fever, we studied on the mechanisms of pyrethroid-insecticide resistance of Aedes aegypti. We focused on (1)Altered target site sensitivity, (2)Reduced penetration of insecticide, and (3)Enhanced metabolic activity, to elucidate the mechanism of the resistance. We found that reduced sensitivity of target site (Vssc) due to amino acid substitutions conferred resistance. Enhanced activity of pyrethroid metabolism by cytochrome P450s was also one of the major factors of the resistance. Further, we identified that both CYP9M6 and CYP6BB2 were capable of metabolizing permethrin and are over produced in SP strain.