Development of evaluation of health effects and environmental carcinogens exposure in molecular epidemiology

多环芳烃（PAHs）是一类含有多种致癌物质的化合物。我们进行了一项横断面研究，钢铁厂工人（n=57），以确定生物标志物，反映致癌性的多环芳烃。采用气相色谱-质谱联用技术测定了15种多环芳烃的个人暴露水平，采用高效液相色谱-荧光检测器和液相色谱-质谱联用技术分析了尿液中的代谢物。检测到11种已知的尿代谢物1-、2-羟基萘、2-羟基芴、1-、2-、3-、9-羟基菲、1-羟基芘、3-羟基苯并[a]蒽、6-羟基蒽、3-羟基苯并[a]芘。总多环芳烃与尿2-羟基萘的相关性最高。在所检测的尿代谢产物中，1-OH芘与“多环芳烃的Sigma致癌效力”（根据个人多环芳烃水平和相对效力因子计算的7种致癌多环芳烃之和）的相关性最大，表明尿1-OH芘是多环芳烃暴露最全面的致癌生物标志物。

Polycyclic aromatic hydrocarbons (PAHs) are multiple compounds which include many carcinogens. We conducted a cross-sectional study in steel plant workers (n=57), to identify biomarkers that reflect the carcinogenicity of PAHs. Level of personal exposure to 15 PAHs was measured using GC-MS. Urinary metabolites analyzed using HPLC with a fluorescence detector and LC-MSMS. Eleven known urinary metabolites 1-, 2-hydroxy(OH)naphthalene, 2-OH fluorene, 1-, 2-, 3-, 9-OH phenanthrene, 1-OH pyrene, 3-OH benz[a]anthracene, 6-OH chrysene, 3-OH benzo[a]pyrene were detected. The highest correlation was between Total-PAHs and urinary 2-OH naphthalene. Among the detected urinary metabolites, 1-OH pyrene was found to the greatest correlation with the "Sigma carcinogenic potency of PAHs" (sum of seven carcinogenic PAHs calculated from levels of personal PAHs and relative potency factors), indicated that urinary 1-OH pyrene was the most comprehensive carcinogenic biomarker of exposure to PAHs.