Research of Inflammatory Bowel Disease Based on Clinical Immunology and Endoscopy

基于临床免疫学和内镜检查的炎症性肠病研究

• 批准号: 23590949
• 负责人: IWAO Yasushi
• 金额: $ 3.33万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590949/
• 关键词: 炎症性腸疾患; alternative Th1; 腸内細菌

In CD4+CD45RBhigh adoptive transfer colitis model, we demonstrated that RORgt+ Th17 and Th17/Th1 cells become colitogenic alternative Th1 cells in vivo. Treg cells suppressed this pathway and development of intestinal inflammation. In addition, we found that Clostridium butyricum, which produces butyrate, suppress intestinal inflammation in mice colitis models. Interestingly, C. butyricum promoted IL-10 production by intestinal macrophage, but not Treg cells. Fecal 16S rRNA analysis, C. butyricum was decreased in the patients with IBD. Our findings provide the new insight into the mechanism of colitogenic T cell development in vivo and suggests the potential of Clostridium butyricum as a novel probiotics.

在CD4+CD45RB高过继转移性结肠炎模型中，我们证明了RORgt+Th17和Th17/Th1细胞在体内成为致病替代Th1细胞。Treg细胞抑制了这一途径和肠道炎症的发展。此外，我们还发现，产生丁酸盐的丁酸梭菌可以抑制小鼠结肠炎模型的肠道炎症。有趣的是，丁酸杆菌能促进肠道巨噬细胞产生IL-10，但不能促进Treg细胞。粪便16S rRNA分析显示，IBD患者粪便中酪氨酸菌减少。我们的发现为体内T细胞发育的机制提供了新的见解，并提示了酪酸梭菌作为一种新型益生菌的潜力。

项目成果

Endoscopic and pathologic changes of the upper gastrointestinal tract in Crohn's disease.

• DOI: 10.1155/2014/610767
• 发表时间: 2014
• 期刊: BioMed research international
• 作者: Sakuraba A;Iwao Y;Matsuoka K;Naganuma M;Ogata H;Kanai T;Hibi T
• 通讯作者: Hibi T

Mucosal healing with oral tacrolimus is associated with favorable medium- and long-term prognosis in steroid-refractory/dependent ulcerative colitis patients.

• DOI: 10.1016/j.crohns.2013.04.018
• 发表时间: 2013-12-01
• 期刊: Journal of Crohn's & colitis
• 作者: Miyoshi, Jun;Matsuoka, Katsuyoshi;Hibi, Toshifumi
• 通讯作者: Hibi, Toshifumi

Dendritic cells administered intrarectally penetrate the intestinal barrier to break intestinal tolerance via Th2-medeiated colitis in mice.

直肠内施用的树突状细胞可穿透肠道屏障，通过 Th2 介导的小鼠结肠炎破坏肠道耐受性。

• DOI: 10.1016/j.imlet.2013.01.010
• 发表时间: 2013
• 期刊: Immunology Letters
• 影响因子: 4.4
• 作者: Handa T;Kanai T;Sato T;Mikami Y;Sujino T;Hayashi. A;Mizuno S;Matsumoto A;Hisamatsu T;Hibi T.
• 通讯作者: Hibi T.

Complete mucosal healing is associated with long-term remission in ulcerative colitis.

粘膜完全愈合与溃疡性结肠炎的长期缓解相关。

• 发表时间: 2012
• 作者: Inoue N;Takabayashi K;Matsuoka K;Hisamatsu T;Kanai T;Ogata H;Iwao Y;Hibi T.
• 通讯作者: Hibi T.

The use of infliximab in the prevention of postsurgical recurrence in polysurgery Crohn's disease patients: a pilot open-labeled prospective study.

使用英夫利昔单抗预防多手术克罗恩病患者的术后复发：一项开放标记的试点前瞻性研究。

• DOI: 10.1007/s00384-011-1398-y
• 发表时间: 2012
• 期刊: Int J Colorectal Dis.
• 作者: Sakuraba A;Sato T;Matsukawa H;Okamoto S;Takaishi H;Ogata H;Iwao Y;Hibi T.
• 通讯作者: Hibi T.