Re-uniting marsupials and eutherians by embryonic micromanipulation

通过胚胎显微操作将有袋动物和真兽动物重新结合

• 批准号: DP0344941
• 负责人: Prof Marilyn Renfree
• 金额: $ 4.44万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0344941
• 关键词: Re; uniting; marsupials; eutherians; embryonic

The unique responsibility for transmitting life from generation to generation normally depends on the gametes. This project will use new reproductive technologies to investigate the properties of the oocyte in reprogramming somatic cell nuclei, and will use the nuclei of both marsupial and eutherian somatic cells to test this. We will also use both marsupial and eutherian genes to insert into the oocyte to create the first transgenic marsupials. We will also investigate the ability of spermatozoa from species of increasing genetic distance to ferttilise marsupial eggs using intracytoplasmic sperm injection (ICSI).

将生命代代相传的独特责任通常取决于配子。该项目将使用新的生殖技术来研究卵母细胞在体细胞核重编程中的特性，并将使用有袋动物和真兽目体细胞的细胞核来测试这一点。我们还将使用有袋动物和真兽目动物的基因插入卵母细胞，以创造第一个转基因有袋动物。 我们还将调查的能力，精子从物种的遗传距离增加受精有袋类卵子使用卵胞浆内单精子注射（ICSI）。