Examination of evidence of causative pathogen in bacterial pneumonia using clone library analysis

我们使用克隆文库法作为一种不依赖培养的方法，使用16S核糖体RNA基因测序分析与传统培养方法相比，评估了诊断为社区获得性肺炎或医疗保健相关性肺炎患者的肺炎病原体。我们的结果表明，相对较高的厌氧菌和口腔细菌的检出率的分子方法，特别是在老年人，比以前的报道。我们推测，我们使用的分子方法是一个有用的工具来检测肺炎的病原体，这些细菌可能在下呼吸道感染中发挥更重要的作用。

We evaluated causative pathogens of pneumonia in patients diagnosed with community-acquired pneumonia or healthcare-associated pneumonia using the clone library method as a cultivation-independent method using 16S ribosomal RNA gene sequencing analysis compared to conventional cultivation methods. Our result showed that relatively higher rates of anaerobes and oral bacteria were detected by the molecular method, especially in the elderly, than previously reported. It is speculated that the molecular method we used is a useful tool for detecting causative pathogens of pneumonia, and these bacteria may play more important roles in lower respiratory tract infections.