Analysis of activation of glomerular parietal epithelial cells to podocyte injury

肾小球壁上皮细胞活化对足细胞损伤的分析

• 批准号: 23591176
• 负责人: SASAKI Satoshi
• 金额: $ 2.75万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591176/
• 关键词: 糸球体硬化症; 慢性腎疾患; 糸球体上皮細胞; 腎臓学; ネフローゼ症候群; 巣状分節性糸球体硬化症

Recent investigations have disclosed that activation of parietal epithelial cells (PECs) plays key roles in the process of focal segmental glomerulosclerosis (FSGS). In this experimental project, we have tried to investigate dynamic behavior of activated PECs in the evolution of FSGS. We conducted serial assessment of activated PECs using the mouse models of lipopolysaccharide(LPS)-induced proteinuria and adriamycin (ADR) nephrosis. To further analyze the role of activated PECs, we induced rapid progressive podocytopathy using LPS-treated ADR nephrosis mice. As the cell markers for activation, we utilized two markers , CD44 and CXCR4. Using models of ADR nephrosis in mice of different ages, we found that CD44+PECs play significant roles in progressive glomerulosclerosis and the prevalence of the cells reflects different degrees of podocyte injury. In addition, analysis of two different markers for PEC activation clarified different roles of these markers during cell activation process.

最近的研究表明，壁上皮细胞（PEC）的激活在局灶节段性肾小球硬化（FSGS）的过程中发挥着关键作用。在这个实验项目中，我们试图研究激活的 PEC 在 FSGS 进化中的动态行为。我们使用脂多糖（LPS）诱导的蛋白尿和阿霉素（ADR）肾病的小鼠模型对活化的 PEC 进行了系列评估。为了进一步分析激活的 PEC 的作用，我们使用 LPS 治疗的 ADR 肾病小鼠诱导快速进行性足细胞病。作为激活的细胞标记，我们使用了两种标记：CD44 和 CXCR4。利用不同年龄小鼠的 ADR 肾病模型，我们发现 CD44+PECs 在进行性肾小球硬化中发挥重要作用，并且细胞的流行程度反映了不同程度的足细胞损伤。此外，对两种不同的 PEC 激活标记物的分析阐明了这些标记物在细胞激活过程中的不同作用。

项目成果

for the Japanese Study Group of Renal Disease in Children. Two-Year Follow-Up of a Prospective Clinical Trial of Cyclosporine for Frequently Relapsing Nephrotic Syndrome in Children.

日本儿童肾病研究小组。

• DOI: 10.2215/cjn.00110112
• 发表时间: 2012
• 期刊: Clin J Am Soc Nephrol
• 作者: Ishikura K;Yoshikawa N;Nakazato H;Sasaki S;Iijima K;Nakanishi K;Matsuyama T;Ito S;Yata N;Ando T;Honda M
• 通讯作者: Honda M

Serial assessment of activated parietal epithelial cells in the mouse model of LPS-induced proteinuria and adriamycin nephrosis

LPS诱导蛋白尿和阿霉素肾病小鼠模型中活化壁上皮细胞的系列评估

• 发表时间: 2013
• 作者: Takeshi Yamazaki;Satoshi Sasaki;Asako Hayashi;Yasuyuki Sato;Takayuki Okamoto;Tadashi Ariga
• 通讯作者: Tadashi Ariga

アドリアマイシン腎症におけるポドサイト喪失に伴う活性化壁側上皮細胞の意義

阿霉素肾病中活化的壁上皮细胞与足细胞丢失的意义

• 发表时间: 2013
• 作者: 山崎健史;佐々木聡;林麻子;佐藤泰征;岡本孝之;有賀正
• 通讯作者: 有賀正

Podocyte expression of nonmuscle myosin heavy chain-IIA decreases in idiopathic nephrotic syndrome, especially in focal segmental glomerulosclerosis.

• DOI: 10.1093/ndt/gft350
• 发表时间: 2013-12
• 期刊: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
• 作者: K. Miura;H. Kurihara;S. Horita;H. Chikamoto;M. Hattori;Y. Harita;Haruko Tsurumi;Yuko Kajiho;Y. Sawada;S. Sasaki;T. Igarashi;S. Kunishima;T. Sekine

Serial assessment of activated parietal epithelial cells in the LPS-induced proteinuria and adriamycin nephrosis

LPS 诱导的蛋白尿和阿霉素肾病中活化的壁上皮细胞的系列评估

• 发表时间: 2013
• 作者: Takeshi Yamazaki;Satoshi Sasaki;Asako Hayashi;Yasuyuki Sato;Takayuki Okamoto;Tadashi Ariga
• 通讯作者: Tadashi Ariga