Analysis of activation of glomerular parietal epithelial cells to podocyte injury

最近的研究表明，壁上皮细胞(PECs)的激活在局灶性节段性肾小球硬化(FSGS)的发病过程中起着关键作用。在这个实验项目中，我们试图研究激活的PECs在FSGS进化过程中的动态行为。我们使用脂多糖(LPS)诱导的蛋白尿和阿霉素(ADR)肾病的小鼠模型对激活的PECs进行了一系列评估。为了进一步分析活化的PECs的作用，我们用脂多糖治疗的ADR肾病小鼠诱导了快速进行性足细胞病变。我们利用CD44和CXCR4两个标记作为激活的细胞标记。利用不同年龄段小鼠的ADR肾病模型，我们发现CD44peS在进行性肾小球硬化中起着重要作用，其发生率反映了足细胞损伤的不同程度。此外，对两种不同的PEC激活标志物的分析阐明了这两种标志物在细胞激活过程中的不同作用。

Recent investigations have disclosed that activation of parietal epithelial cells (PECs) plays key roles in the process of focal segmental glomerulosclerosis (FSGS). In this experimental project, we have tried to investigate dynamic behavior of activated PECs in the evolution of FSGS. We conducted serial assessment of activated PECs using the mouse models of lipopolysaccharide(LPS)-induced proteinuria and adriamycin (ADR) nephrosis. To further analyze the role of activated PECs, we induced rapid progressive podocytopathy using LPS-treated ADR nephrosis mice. As the cell markers for activation, we utilized two markers , CD44 and CXCR4. Using models of ADR nephrosis in mice of different ages, we found that CD44+PECs play significant roles in progressive glomerulosclerosis and the prevalence of the cells reflects different degrees of podocyte injury. In addition, analysis of two different markers for PEC activation clarified different roles of these markers during cell activation process.