CCDC3 is specifically upregulated in omental adipose tissue in subjects with abdominal obesity

为了寻找内脏肥胖的新标记物，我们采集了43名日本男性的内脏和皮下脂肪组织。我们使用从五名腹型肥胖男性和五名非肥胖男性的内脏和皮下脂肪组织中获得的RNA进行了微阵列分析。CCDC3(编码卷曲结构域蛋白3)在腹型肥胖者的大网膜脂肪组织中表达上调(3.07倍)，而在皮下脂肪组织中的表达上调(0.89倍)。在两种不同的肥胖小鼠模型中也发现了相似的表达模式。在对所有43名男性的分析中，大网膜中的CCDC3mRNA水平与腰围和BMI呈正相关，而皮下脂肪组织中的CCDC3mRNA水平与腰围和BMI呈正相关。Western blotting证实CCDC3是一种分泌型蛋白，提示CCDC3是一种潜在的内脏肥胖症的生物标志物。

To search for novel markers of visceral adiposity, visceral and subcutaneous adipose tissues were obtained from 43 Japanese men. We conducted microarray analysis using RNA from visceral and subcutaneous adipose tissues obtained from five men with abdominal obesity and five non-obese men. The mRNA expression of CCDC3 (encoding coiled-coil domain-containing protein 3) was upregulated in omental adipose tissues from abdominally obese subjects (3.07-fold) but not in subcutaneous adipose tissues (0.89-fold). Similar expression patterns were found in two distinct mouse models of obesity. In the analysis of all 43 men, CCDC3 mRNA levels in omental, but not in subcutaneous adipose tissue, were positively correlated with waist circumference and BMI. CCDC3 was predicted to be a secretory protein, which was confirmed by western blotting.These results suggest that CCDC3 is a potential biomarker for estimating visceral adiposity.