Mining of novel granin-derived bioactive peptides that regulate insulin secretion.

为了探讨NERP-2在胰腺中的生理作用，我们检测了它对胰岛素分泌的影响。我们在胰岛检测到NERP-2免疫反应性，在那里它与胰岛素广泛共存。NERP-2以剂量依赖方式增加小鼠胰岛葡萄糖刺激的胰岛素分泌(GSIS)。给药后，NERP-2可显著提高GSIS。NERP-1或NERP-2-Gly(非酰胺化NERP-2)均不能刺激胰岛素分泌。钙离子成像分析显示，NERP-2增加了MIN6细胞的钙内流。这些发现表明NERP-2是一种新的刺激胰岛素分泌的生物活性多肽。

To explore the physiological roles of NERP-2 in the pancreas, we examined its effects on insulin secretion. We detected NERP-2 immunoreactivity in pancreatic islets, where it co-localized extensively with insulin. NERP-2 increased glucose-stimulated insulin secretion (GSIS) in a dose-dependent manner from isolated mouse pancreatic islets. NERP-2 significantly augmented GSIS after administration to rodents. Neither NERP-1 nor NERP-2-Gly (non-amidated NERP-2) stimulated insulin secretion. Calcium-imaging analysis demonstrated that NERP-2 increased the calcium influx in MIN6 cells. These findings show that NERP-2 is a novel bioactive peptide to stimulate insulin secretion.