Mining of novel granin-derived bioactive peptides that regulate insulin secretion.

为了探索NERP-2在胰腺中的生理作用，我们检测了其对胰岛素分泌的影响。我们在胰岛中检测到NERP-2免疫反应性，在那里它与胰岛素广泛共定位。NERP-2以剂量依赖性方式增加来自分离的小鼠胰岛的葡萄糖刺激的胰岛素分泌（GSIS）。NERP-2显著增强啮齿动物给药后的GSIS。NERP-1和NERP-2-Gly（非酰胺化NERP-2）均不刺激胰岛素分泌。钙成像分析表明，NERP-2增加了MIN 6细胞的钙内流。这些发现表明NERP-2是一种新的刺激胰岛素分泌的生物活性肽。

To explore the physiological roles of NERP-2 in the pancreas, we examined its effects on insulin secretion. We detected NERP-2 immunoreactivity in pancreatic islets, where it co-localized extensively with insulin. NERP-2 increased glucose-stimulated insulin secretion (GSIS) in a dose-dependent manner from isolated mouse pancreatic islets. NERP-2 significantly augmented GSIS after administration to rodents. Neither NERP-1 nor NERP-2-Gly (non-amidated NERP-2) stimulated insulin secretion. Calcium-imaging analysis demonstrated that NERP-2 increased the calcium influx in MIN6 cells. These findings show that NERP-2 is a novel bioactive peptide to stimulate insulin secretion.