Functional analysis of phospholipase Cδ3 in inner hair cells

内毛细胞磷脂酶Cδ3的功能分析

• 批准号: 20770108
• 负责人: YAMAGUCHI Hideki
• 金额: $ 2.75万
• 依托单位: Tokyo University of Pharmacy and Life Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770108/
• 关键词: 細胞情報伝達機構; イノシトールリン脂質代謝; ホスホリパーゼC; ミオシン; 内耳有毛細胞; エンドサイトーシス; 微絨毛形成; イノシトールリン脂質

In this study, we performed functional analyses of phospholipase Cδ3, an enzyme involved in phosphoinositide metabolism, and its binding protein Myosin VI, a motor protein involved in molecular transport. As a result, we revealed that these proteins are coexpressed in the inner hair cells in mice and colocalized on the plasma membrane in cultured cells. Furthermore, we found that phospholipase Cδ3 regulates several cellular processes which depends on Myosin VI function, such as endocytosis and microvilli formation.

在这项研究中，我们进行了功能分析的磷脂酶Cδ3，参与磷酸肌醇代谢的酶，和它的结合蛋白肌球蛋白VI，参与分子运输的马达蛋白。结果表明，这些蛋白质在小鼠内毛细胞中共表达，在培养细胞中共定位于质膜上。此外，我们还发现磷脂酶Cδ3调节着几个依赖于肌球蛋白VI功能的细胞过程，如内吞作用和微绒毛形成。

项目成果

Phosphatidylinositol 4,5-bisphosphate and PIP5-kinase Iα are required for invadopodia formation in human breast cancer cells

人乳腺癌细胞侵袭伪足的形成需要磷脂酰肌醇 4,5-二磷酸和 PIP5 激酶 Iα

• 发表时间: 2010
• 期刊: Cancer Sci. (In press)
• 作者: 芝崎 太;内野博之;Tanaka M;Yamaguchi H
• 通讯作者: Yamaguchi H

Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation.

• DOI: 10.1083/jcb.200812176
• 发表时间: 2009-08-24
• 期刊: The Journal of cell biology
• 作者: Oser M;Yamaguchi H;Mader CC;Bravo-Cordero JJ;Arias M;Chen X;Desmarais V;van Rheenen J;Koleske AJ;Condeelis J
• 通讯作者: Condeelis J

MyosinVIの膜輸送機能におけるホスフォリパーゼCδ3の関与

磷脂酶 Cδ3 参与肌球蛋白 VI 的膜转运功能

• 发表时间: 2008
• 作者: 櫻井和之;平田真之;石井里佳;中村由和;河内全;山口英樹;深見希代
• 通讯作者: 深見希代

Membrane targeting of WAVE2 is not sufficient for WAVE2-dependent actin polymerization: a role for IRSp53 in mediating the interaction between Rac and WAVE2

• DOI: 10.1242/jcs.010272
• 发表时间: 2008-02-01
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Abou-Kheir, Wassim;Isaac, Beth;Cox, Dianne
• 通讯作者: Cox, Dianne

A Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasis.

• DOI: 10.1016/j.devcel.2008.09.003
• 发表时间: 2008-12
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Philippar, Ulrike;Roussos, Evanthia T.;Oser, Matthew;Yamaguchi, Hideki;Kim, Hyung-Do;Giampieri, Silvia;Wang, Yarong;Goswami, Sumanta;Wyckoff, Jeffrey B.;Lauffenburger, Douglas A.;Sahai, Erik;Condeelis, John S.;Gertler, Frank B.
• 通讯作者: Gertler, Frank B.