Analysis of leukemic microenvironment in RUNX1/EVI1 mouse model

RUNX1/EVI1小鼠模型白血病微环境分析

• 批准号: 23591406
• 负责人: NAKAMURA Yuka
• 金额: $ 3.33万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591406/
• 关键词: 白血病モデルマウス; 白血病幹細胞; 骨髄微小環境; ニッチ因子

Mice transplanted with RUNX1/EVI1-expressed bone marrow cells showed increased number of platelets and splenomegaly. In these mice increased number of megakaryocyte was recognized in bone marrow and spleen. RUNX1/EVI1 enhanced colony replating activity of hematopoietic cells. The expression level of Skp2, which is one of a target genes of RUNX1/EVI1, was decreased in colony forming cells gradually. Some mice developed leukemia after transplantation. c-Kit-positive leukemic cells invaded bone marrow, spleen and liver.

移植RUNX 1/EVI 1表达的骨髓细胞的小鼠显示血小板数量增加和脾肿大。在这些小鼠中，骨髓和脾脏中巨核细胞数量增加。RUNX 1/EVI 1增强造血细胞集落再铺板活性。RUNX 1/EVI 1的靶基因之一Skp 2在集落形成细胞中的表达水平逐渐降低。一些小鼠在移植后发生白血病。c-Kit阳性白血病细胞侵入骨髓、脾和肝。

项目成果

Enhanced Angpt1/Tie2 signaling affects the differentiation and long-term repopulation ability of hematopoietic stem cells

• DOI: 10.1016/j.bbrc.2012.11.002
• 发表时间: 2013-01-04
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ikushima, Yoshiko Matsumoto;Arai, Fumio;Suda, Toshio
• 通讯作者: Suda, Toshio

Acute myeloid leukemia with t(7;21)(q11.2;q22) expresses a novel, reversed-sequence RUNX1-DTX2 chimera

• DOI: 10.1007/s12185-012-1112-z
• 发表时间: 2012-08-01
• 期刊: INTERNATIONAL JOURNAL OF HEMATOLOGY
• 影响因子: 2.1
• 作者: Maki, Kazuhiro;Sasaki, Ko;Mitani, Kinuko
• 通讯作者: Mitani, Kinuko

Fadd and Skp2 are possible downstream targets of RUNX1-EVI1

Fadd 和 Skp2 可能是 RUNX1-EVI1 的下游目标

• 发表时间: 2013
• 期刊: Int J Hematol
• 影响因子: 2.1
• 作者: Maki K;Sugita F;Nakamura Y;Sasaki K;Mitani K
• 通讯作者: Mitani K

Overexpression of MIR9 indicates poor prognosis in acute lymphoblastic leukemia

• DOI: 10.3109/10428194.2013.790023
• 发表时间: 2014-01-01
• 期刊: LEUKEMIA & LYMPHOMA
• 影响因子: 2.6
• 作者: Sugita, Fusako;Maki, Kazuhiro;Mitani, Kinuko
• 通讯作者: Mitani, Kinuko