Role of Na/Ca exchanger on vascular tone of fetal and neonatal ductus arteriosus and peripheral pulmonary artery

(1)在肺动脉和动脉导管中均可见Na/Ca交换体亚型（Slc 8A 1、A2、A3）。在A1和A3中观察到高表达水平，而A2表达水平较低。(2)在发育过程中，Slc 8A 1在动脉导管和肺动脉中的表达有轻微下降的趋势，而在心室肌中则有明显的变化。另一方面，Slc 8A 3倾向于随着发育而增加。免疫组化染色显示，动脉导管和肺动脉中A3强染色，其次是A1。Slc 8A 2呈阴性。(4)将Slc 8A 1转染到HEK 293细胞中。(5)用膜片钳法测定Slc 8A 1细胞的Na/Ca交换电流。然而，在各种条件下（如缺氧）的电流测量尚未完成。将继续进行研究。

(1) All isoform of Na/Ca exchanger member (Slc8A1, A2, A3) was seen in pulmonary artery and ductus arterisus. High expression levels were observed in A1 and A3, whereas A2 expression level was low. (2) In developmental changes, Slc8A1 tends to decrease slightly in ductus arteriosus and pulmonary artery, compare with significant change is observed in ventricular muscle. On the other hand, Slc8A3 tends to increase with development. In immunostaining analyses showed strong stain in A3 followed in A1 in ductus arteriosus and pulmonary artery. Slc8A2 showed negative immunostaining. (4) Transfection of Slc8A1 into HEK293 cell was performed. (5) Measurement of the Na/Ca exchanger current of Slc8A1 was performed by patch clamp method. However, measurements of the currents under various conditions such as hypoxia were not completed. Research will be continued.