Role of Na/Ca exchanger on vascular tone of fetal and neonatal ductus arteriosus and peripheral pulmonary artery

(1) Na/Ca交换体Slc8A1、A2、A3在肺动脉和动脉导管中均可见。A1和A3高表达，A2低表达。(2)在发育变化中，Slc8A1在动脉导管和肺动脉中有轻微下降的趋势，而在心室肌中有明显变化。另一方面，随着发育，Slc8A3有增加的趋势。免疫染色分析显示，动脉导管和肺动脉的A3强染色，A1强染色。Slc8A2免疫染色呈阴性。(4)将Slc8A1转染HEK293细胞。(5)采用膜片钳法测量Slc8A1的Na/Ca交换电流。然而，在缺氧等各种条件下的电流测量还没有完成。研究将继续进行。

(1) All isoform of Na/Ca exchanger member (Slc8A1, A2, A3) was seen in pulmonary artery and ductus arterisus. High expression levels were observed in A1 and A3, whereas A2 expression level was low. (2) In developmental changes, Slc8A1 tends to decrease slightly in ductus arteriosus and pulmonary artery, compare with significant change is observed in ventricular muscle. On the other hand, Slc8A3 tends to increase with development. In immunostaining analyses showed strong stain in A3 followed in A1 in ductus arteriosus and pulmonary artery. Slc8A2 showed negative immunostaining. (4) Transfection of Slc8A1 into HEK293 cell was performed. (5) Measurement of the Na/Ca exchanger current of Slc8A1 was performed by patch clamp method. However, measurements of the currents under various conditions such as hypoxia were not completed. Research will be continued.