Role of Na/Ca exchanger on vascular tone of fetal and neonatal ductus arteriosus and peripheral pulmonary artery

Na/Ca交换器对胎儿和新生儿动脉导管和外周肺动脉血管张力的作用

• 批准号: 23591605
• 负责人: KATSUBE YASUHIRO
• 金额: $ 3.33万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591605/
• 关键词: Na/Ca交換輸送; 血管平滑筋; 動脈管; 胎児; 収縮

(1) All isoform of Na/Ca exchanger member (Slc8A1, A2, A3) was seen in pulmonary artery and ductus arterisus. High expression levels were observed in A1 and A3, whereas A2 expression level was low. (2) In developmental changes, Slc8A1 tends to decrease slightly in ductus arteriosus and pulmonary artery, compare with significant change is observed in ventricular muscle. On the other hand, Slc8A3 tends to increase with development. In immunostaining analyses showed strong stain in A3 followed in A1 in ductus arteriosus and pulmonary artery. Slc8A2 showed negative immunostaining. (4) Transfection of Slc8A1 into HEK293 cell was performed. (5) Measurement of the Na/Ca exchanger current of Slc8A1 was performed by patch clamp method. However, measurements of the currents under various conditions such as hypoxia were not completed. Research will be continued.

(1) Na/Ca交换体Slc8A1、A2、A3在肺动脉和动脉导管中均可见。A1和A3高表达，A2低表达。(2)在发育变化中，Slc8A1在动脉导管和肺动脉中有轻微下降的趋势，而在心室肌中有明显变化。另一方面，随着发育，Slc8A3有增加的趋势。免疫染色分析显示，动脉导管和肺动脉的A3强染色，A1强染色。Slc8A2免疫染色呈阴性。(4)将Slc8A1转染HEK293细胞。(5)采用膜片钳法测量Slc8A1的Na/Ca交换电流。然而，在缺氧等各种条件下的电流测量还没有完成。研究将继续进行。

项目成果

動脈管・肺動脈の発達に伴う Na+/Ca2+交換輸送体mRNAの発現変動

与动脉导管和肺动脉发育相关的Na+/Ca2+交换转运蛋白mRNA表达变化

• 发表时间: 2012
• 作者: 羽山恵美子;勝部康弘;中西敏雄;小川俊一
• 通讯作者: 小川俊一