Interstitial pathophysiology and angiogenesis and its imaging in myocardial ischemia and remodeling

心肌缺血和重塑中的间质病理生理学和血管生成及其成像

• 批准号: 23591756
• 负责人: TAKI Junichi
• 金额: $ 3.24万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591756/
• 关键词: 心筋虚血; 心筋梗塞; メチオニン; 血管新生; リモデリング; マクロファージ; 心筋繊維芽細胞; テネイシンC; 心筋線維芽細胞; Galactro-RGD

We investigated the serial changes and mechanisms of C-14-methionine uptake in a rat model of myocardial ischemia and reperfusion. Both C-14-methionine and Tl-201 uptake were reduced in the area at risk at 1 day after reperfusion. However, 3 days after reperfusion, an increased C-14-methionine uptake was observed corresponding to the area of still reduced Tl-201 uptake, and the C-14-Methionine uptake gradually declined until 28 days. The C-14-methionine uptake area at 3-7 days corresponded well to the macrophage infiltrations demonstrated by positive CD68 staining. Anti alpha-SMA staining appeared at 7 days, after which CD68 staining was gradually replaced by the SMA staining, suggesting that methionine uptake in the early phase after ischemia and reperfusion might reflect inflammatory activity. Methionine imaging may be useful for inflammatory imaging early after myocardial infarction.

我们研究了大鼠心肌缺血再灌注模型中c -14-蛋氨酸摄取的系列变化及其机制。再灌注后1天，危险区域的c -14-蛋氨酸和Tl-201摄取均减少。然而，在再灌注3天后，与仍减少的Tl-201摄取面积相对应的c -14-蛋氨酸摄取增加，并且c -14-蛋氨酸摄取逐渐下降，直到28天。3-7天的c -14-蛋氨酸摄取面积与CD68阳性染色显示的巨噬细胞浸润相吻合。第7天出现抗α -SMA染色，之后CD68染色逐渐被SMA染色所取代，提示缺血再灌注后早期蛋氨酸摄取可能反映了炎症活性。蛋氨酸显像可用于心肌梗死后早期的炎症显像。