Novel therapeutic starategy for CEACAM1 expressing colorectal cancer cells beyond the invasion front

点击翻译按钮获取中文摘要

Enhanced CEACAM-4S (short cytoplasmic isoform) induced CD44 expression, incresed ALDH1 activity, and anchorage indeoendent growth of colorectal cancer cells compared with vector control, suggesting that a possibility that CEACAM1-4S induce CSC property. In clinical setting, Overexpression of CEACAM1-S (short cytoplasmic isoform) at the invasion fronat of colorectal cancer tissue indicated poor survival of patients. By addition of 5FU to colorectal cancer cells on plastic, CEACAM1-4L transfected HT29 cells significantly survived in comparison with CEACAM1-4S or vector control transfected cells. In 3D culture, addition of 5FU induced apoptotic burst of colorectal cancer sphere formed by CEACAM1-4S. A multivariate analysis for recurrence after curative surgery and adjuvant chemotherapy for Stage III colorectal cancer patients showed that the hollow spheroid formation beyond the invasive front is an independent risk factor.