In vivo real-time visualization of in inflammatory bowel disease model mice using two photon laser scanning microscopy

使用双光子激光扫描显微镜对炎症性肠病模型小鼠进行体内实时可视化

• 批准号: 23591958
• 负责人: UCHIDA Keiichi
• 金额: $ 3.41万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591958/
• 关键词: 潰瘍性大腸炎; 二光子レーザー顕微鏡; DSS腸炎マウス; 炎症性腸疾患

In this study, we attempted to visualize in vivo therapeutic response of infliximab (IFX) in the murine UC model using two photon laser scanning microscopy (TPLSM) and to analyze their histopathological response morphologically. Both extensive inflammatory cell infiltration and the damage or loss of epithelial cells in the crypt was observed in vivo real-time in the dextran sodium sulfate (DSS) colitis model of green fluorescent protein (GFP) expressing mice using TPLSM. The number of leukocyte infiltrates within the crypt (2.4 versus 6 per field of view: FOV) and the number of crypt loss (1.0 versus 4.5 per FOV) were reduced in IFX-treated mice, compared with non-IFX, control mice. The length of colonic crypt (99.8 versus 80.0 micrometer) was increased in IFX-treated mice, compared with non-IFX, control mice. Serial change in regenerative epithelial cells was not observed, which regenerated from the bottom of the crypt toward the top of the crypt (the luminal side).

在本研究中，我们试图用双光子激光扫描显微镜(TPLSM)观察英夫利昔单抗(ifliximab，IFX)对小鼠UC模型的体内治疗作用，并对其组织病理学反应进行形态学分析。用TPLSM实时观察表达绿色荧光蛋白(GFP)的小鼠葡聚糖硫酸钠(DSS)结肠炎模型体内广泛的炎性细胞浸润和隐窝内上皮细胞的损伤或丢失。与非IFX对照组小鼠相比，IFX治疗组小鼠隐窝内白细胞渗入的数量(2.4个/视野：FOV)和隐窝丢失的数量(1.0个/FOV比4.5个/视野)均减少。与非IFX对照组小鼠相比，IFX处理组小鼠的结肠隐窝长度(99.8微米比80.0微米)增加。没有观察到再生上皮细胞的连续变化，再生上皮细胞是从隐窝底部向隐窝顶部(腔侧面)再生的。

项目成果

In vivo real-time imaging of chemotherapy response on the liver metastatic tumor microenvironment using multiphoton microscopy.

使用多光子显微镜对肝转移肿瘤微环境的化疗反应进行体内实时成像。

• DOI: 10.3892/or.2012.1983
• 发表时间: 2012
• 期刊: Oncol Rep.
• 作者: Nagai Y;et al;Kimura N;Tang W;Tanaka K et al.
• 通讯作者: Tanaka K et al.

Intravital imaging of gastrointestinal diseases in preclinical models using two-photon laser scanning microscopy.

使用双光子激光扫描显微镜在临床前模型中对胃肠道疾病进行活体成像。

• DOI: 10.1007/s00595-012-0283-9
• 发表时间: 2013
• 期刊: Surg Today.
• 作者: Tanaka K;Toiyama Y;Inoue Y;Uchida K;Araki T;Mohri Y;Mizoguchi A;Kusunoki M.
• 通讯作者: Kusunoki M.

Intravital dual-colored visualization of colorectal liver metastasis in living mice using two photon laser scanning microscopy

• DOI: 10.1002/jemt.21059
• 发表时间: 2012-03-01
• 期刊: MICROSCOPY RESEARCH AND TECHNIQUE
• 影响因子: 2.5
• 作者: Tanaka, Koji;Morimoto, Yuhki;Kusunoki, Masato
• 通讯作者: Kusunoki, Masato