In vivo real-time visualization of in inflammatory bowel disease model mice using two photon laser scanning microscopy

在这项研究中，我们试图用双光子激光扫描显微镜（TPLSM）观察英夫利昔单抗（IFX）在小鼠UC模型中的体内治疗反应，并从形态学上分析其组织病理反应。用TPLSM建立表达绿色荧光蛋白（GFP）的小鼠右旋糖酐硫酸钠（DSS）结肠炎模型，实时观察小鼠体内炎症细胞的广泛浸润和隐窝上皮细胞的损伤或丢失。与未接受ifx治疗的对照组小鼠相比，ifx治疗小鼠的隐窝内白细胞浸润数量（每视场视野2.4个vs每视场视野6个）和隐窝丢失数量（每视场视野1.0个vs每视场视野4.5个）均有所减少。与非ifx对照小鼠相比，ifx处理小鼠的结肠隐窝长度（99.8微米比80.0微米）增加。未观察到再生上皮细胞的系列变化，其从隐窝底部向隐窝顶部（管腔侧）再生。

In this study, we attempted to visualize in vivo therapeutic response of infliximab (IFX) in the murine UC model using two photon laser scanning microscopy (TPLSM) and to analyze their histopathological response morphologically. Both extensive inflammatory cell infiltration and the damage or loss of epithelial cells in the crypt was observed in vivo real-time in the dextran sodium sulfate (DSS) colitis model of green fluorescent protein (GFP) expressing mice using TPLSM. The number of leukocyte infiltrates within the crypt (2.4 versus 6 per field of view: FOV) and the number of crypt loss (1.0 versus 4.5 per FOV) were reduced in IFX-treated mice, compared with non-IFX, control mice. The length of colonic crypt (99.8 versus 80.0 micrometer) was increased in IFX-treated mice, compared with non-IFX, control mice. Serial change in regenerative epithelial cells was not observed, which regenerated from the bottom of the crypt toward the top of the crypt (the luminal side).