The research that aims at the establishment of an efficient ovarian tissue cryopreservation and transplantation method - For the young female cancer patients

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The effectiveness of cryopreservation for the primordial follicles in the ovarian tissue and the safety of an open-type device of ovarian tissue cryopreservation using vitrificaton technique should be necessary to evaluate as a new technology of ovarian tissue cryopreservation. On the other hand, there is no method to evaluate the success or failure of the ovarian tissue transplantation, and, as for clarifying the distribution of a new blood vessel flowing into the transplant ovarian tissues, it can be with information important at all.In this study, we succeeded to prepare the closed-type device of ovarian tissue cryopreservation using vitrificaton technique that assumed a polymeric film and, on the other hand, evaluated the effectiveness of cryopreservation for the primordial follicles in the ovarian tissue using Rapid-i kit. Furthermore, we succeeded in catching a re-inflow of the bloodstream by echo-wave video diagnosis with the rat fresh ovary.