Analysis of the kinocilia influence in the cochlea outer hair cells

由于C3H/HeJ小鼠的耳蜗外毛细胞动丝中含有瘦素受体，因此我们对已知的糖尿病模型Lepr db/db小鼠进行了分析。与对照组相比，DPOAEs组ABR阈值基本相同，但可接受早期衰减，其听力损失的主要原因是外毛细胞。DPOAEs未见立体纤毛移位，未见毛细胞束转化，但胞浆内有PAS染色的异常滤泡，提示DPOAEs衰减是由于外毛细胞体紊乱而不是静纤毛功能障碍所致。另一方面，瘦素受体完整的高血糖状态C3H/HeJ小鼠ABR阈值和DPOAEs与对照组相近，胞浆内未见异常的PAS染色卵泡。综上所述，外毛细胞动丝缺失与胞浆异常有关，仅在单纯性高血糖状态下无影响。

Because C3H/HeJ mice have leptin receptor in their cochlear outer hair cell kinocilia, we analyze leptin receptor knock out Lepr db/db mice, which were known to model of diabetes mellitus. ABR thresholds were proximately same but accepted early attenuation in DPOAEs compared with control one, the responsible part of their hearing loss was regarded as outer hair cells. There were no stereocilia displacement and no hair cell bundle transformation but abnormal PAS stained follicles in cytoplasm, these meant DPOAEs attenuation were due to outer hair cell body disorder rather than stereocilia disfunction. On the other hand, in leptin receptor intact hyperglycosemia state C3H/HeJ mice, ABR thresholds and DPOAEs were proximately same compared with control one and abnormal PAS stained follicles in cytoplasm were never seen these mice. In conclusion, Absence of outer hair cell kinocilia were related to cytoplasmic abnormality and there ware no affection only in a simple hyperglycosemia state.