Focus on mechanism of chronic ocular graft-vessus-host disease caused by donor-derived mesenchymal stem cells toward development of new treatment.

慢性眼部移植物抗宿主病（GVHD），即慢性GVHD相关性干眼，是同种异体造血干细胞移植后的主要并发症。我们发现，供体来源的间充质干细胞在慢性眼GVHD纤维化的潜在机制中起着重要作用。我们发现供体间充质干细胞移植了GVHD靶器官，如泪腺、结膜、唾液腺、肺、皮肤、肝脏和肠道。我们还认为，供体间充质干细胞与致病区域的T细胞相互作用，促进了cGVHD的发病和这种疾病的慢性。我们进一步发现供体间充质干细胞增加IL-6导致外周血中Tregs减少，反过来增加th17细胞。我们的研究结果表明，供体间充质干细胞触发T细胞的免疫反应和免疫介导的慢性眼移植物抗宿主病纤维化。

Chronic ocular graft-versus-host disease(GVHD), i.e. chronic GVHD related dry eye, is a major complication after allogeneic hematopoietic stem cell transplantation. We have found that donor-derived mesenchymal stem cells play a significant role for underlying mechanism of chronic ocular GVHD fibrosis. We have found donor MSCs engrafted GVHD target organs such as lacrimal gland, conjunctiva, salivary gland, lung, skin, liver and intestine. We also suggested that donor MSCs interacted with T cells in the pathogenic regions and contributed to the onset of cGVHD and chronicity of this disease. We further showed donor MSCs increased IL-6 leading to decreased Tregs and conversely an increase in Th 17 cells in peripheral blood. Our results show that donor MSCs trigger immune responses in T cells and immune mediated fibrosis of chronic ocular GVHD.