A study for intraocular molecular mechanism and functional expression of tissue stem cells induced injury to the retina.

组织干细胞诱导视网膜损伤的眼内分子机制和功能表达的研究。

• 批准号: 23592610
• 负责人: FUKUSHIMA Mikiko
• 金额: $ 3.33万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592610/
• 关键词: 再生医学; 細胞・組織; 生理活性分子; 眼科学; 眼発生・再生医学; 眼細胞生物学

This study was related to the control mechanism differentiation of retinal tissue stem cells and was analyzed by using a sample eye disease and retinal injury animal model. It was possible to suppress glial differentiation of neural stem cells transplanted as well as the expression of CNTF belonging to the IL-6 superfamily is an inflammatory cytokine by a non-steroidal anti-inflammatory agent administered in the retinal injury animal model. Increased expression inflammatory cytokines, growth factors, of amyloid-related proteins was revealed cataract, glaucoma, and retinal disease in specific in the analysis using a sample eye disease. In retinal pigment epithelial cell culture analysis, the transformation-induced effect on the vitreous humor with retinal detachment, epiretinal membrane disease, involvement of phosphorylation signal smad, of NF-kB has been revealed.

本研究涉及视网膜组织干细胞分化的调控机制，并通过使用样本眼病和视网膜损伤动物模型进行分析。通过在视网膜损伤动物模型中施用非甾体抗炎剂，可以抑制移植的神经干细胞的胶质分化以及属于IL-6超家族的CNTF（一种炎性细胞因子）的表达。在使用样本眼部疾病的分析中，白内障、青光眼和视网膜疾病的淀粉样蛋白相关蛋白的炎性细胞因子、生长因子的表达增加。在视网膜色素上皮细胞培养分析中，已经揭示了对具有视网膜脱离、视网膜前膜疾病的玻璃体液的转化诱导作用，NF-κ B的磷酸化信号smad的参与。

项目成果

Dynamic imaging of axonal transport in living retinal ganglion cells in vitro.

体外活体视网膜神经节细胞轴突运输的动态成像。

• DOI: 10.1167/iovs.10-6435
• 发表时间: 2011
• 期刊: Invest Ophthalmol Vis Sci.
• 作者: Takihara Y;Inatani M;Hayashi H;Adachi N;Iwao K;Inoue T;Iwao M;Tanihara H.
• 通讯作者: Tanihara H.

Angptl2 Knockout Mice Suppres Inflammation in Retinal Vessel Induced by High-Fat Diet

Angptl2 基因敲除小鼠可抑制高脂肪饮食引起的视网膜血管炎症

• 发表时间: 2012
• 作者: Ito Y;Oike Y;Tanihara H
• 通讯作者: Tanihara H

Angptl2 Knockout Mice Suppress Inflammation In Retinal Vessels Induced By A High-fat Diet.

Angptl2 基因敲除小鼠可抑制高脂肪饮食引起的视网膜血管炎症。

• 作者: Ito Y;Oike Y;Tanihara H.
• 通讯作者: Tanihara H.

Epithelial Mesenchymal Transition and Human Vitreous Samples in 2D and 3D culture of ARPE-19 cells

ARPE-19 细胞 2D 和 3D 培养中的上皮间质转化和人玻璃体样品

• 发表时间: 2013
• 作者: 高橋枝里;猪俣泰也;川路隆博
• 通讯作者: 川路隆博

Adiponectin Suppresses Adherent Leukocytes in Retinal Vessels Induced by High-Fat Diet

脂联素抑制高脂肪饮食诱导的视网膜血管中的粘附白细胞

• 发表时间: 2011
• 作者: Ito Y;Nakao S;Zandi S;Tanihara H.
• 通讯作者: Tanihara H.