regulatory mechanisms underlying proliferation, differentiation, migration and integration in transplanted retinal stem cells in diseased retinas

病变视网膜移植视网膜干细胞增殖、分化、迁移和整合的调控机制

• 批准号: 18591927
• 负责人: FUKUSHIMA Mikiko
• 金额: $ 2.51万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591927/
• 关键词: Retinal transplantation; Regenerative medicine; Stem cell

The development of retinal regenerative therapy and its efforts to restore visual function in patients with significant visual impairment has recently drawn much attention. Our knowledge of regulatory mechanisms underlying proliferation, differentiation, migration and integration in transplanted progenitors in diseased retinas is far from satisfactory. We introduce our experiments and outline the hopes and problems of cell therapy for damaged and diseased retinas. One of our experiences effectively utilizes stable cell proliferation to prepare a sufficient amount of stem cell pool to establish a stem cell therapy.Wnt signaling may provide a novel strategy for stem cell therapy using adult retinal progenitors prepared from the ciliary margin. Furthermore, transplantation of progenitor cells into the damaged retina revealed that conditions in the host retina affect the fate of transplanted cells through the gp130-signaling pathway. Manipulating host microenvironments may therefore support the proper differentiation of transplanted progenitor cells into the retina for regenerative therapy.

视网膜再生治疗的发展及其在恢复严重视力障碍患者视功能方面的努力最近引起了人们的广泛关注。我们对病变视网膜中移植的祖细胞的增殖、分化、迁移和整合的调控机制的认识还远不能令人满意。我们介绍了我们的实验，并概述了细胞治疗受损和患病视网膜的希望和问题。我们的经验之一是有效地利用稳定的细胞增殖来制备足够量的干细胞库以建立干细胞治疗。Wnt信号可能为利用从睫状缘制备的成人视网膜祖细胞进行干细胞治疗提供新的策略。此外，将祖细胞移植到受损的视网膜中揭示了宿主视网膜中的条件通过gp130信号通路影响移植细胞的命运。因此，操纵宿主微环境可以支持移植的祖细胞适当分化到视网膜中用于再生治疗。

项目成果

Activation of canonical Wnt pathway promotes proliferation of retinal stem cells derived from adult mouse ciliary margin

• DOI: 10.1634/stemcells.2005-0124
• 发表时间: 2006-01-01
• 期刊: STEM CELLS
• 影响因子: 5.2
• 作者: Inoue, T;Kagawa, T;Taga, T
• 通讯作者: Taga, T

Y-27632, a Rho-associated protein kinase inhibitor, attenuates neuronal cell death after transient retinal ischemia

• DOI: 10.1007/s00417-007-0666-6
• 发表时间: 2008-01-01
• 期刊: GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
• 影响因子: 2.7
• 作者: Hirata, Akira;Inatani, Masaru;Tanihara, Hidenobu
• 通讯作者: Tanihara, Hidenobu

HIPKs are involved in regulation of retinal cell number and lens morphology

HIPKs 参与视网膜细胞数量和晶状体形态的调节

• 发表时间: 2006
• 作者: Kawaji;T;Inoue T
• 通讯作者: Inoue T

Pitavastatin: Protection against neuronal retinal damage induced by ischemia-reperfusion injury in rats

• DOI: 10.1080/02713680701649603
• 发表时间: 2007-11-01
• 期刊: CURRENT EYE RESEARCH
• 影响因子: 2
• 作者: Kawaji, Takahiro;Inomata, Yasuya;Tanihara, Hidenobu
• 通讯作者: Tanihara, Hidenobu

hioredoxin inhibits NMDA-induced neurotoxicity in the rat retina

硫氧还蛋白抑制 NMDA 诱导的大鼠视网膜神经毒性

• 发表时间: 2006
• 期刊: J Neurochem. 98・2
• 作者: Inomata Y;et. al.
• 通讯作者: et. al.