Development of ocular irritancy test using cultured corneal epithelial sheet

体内Draize眼试验已成为眼刺激的国际标准测定法，但由于伦理和科学原因，经常受到批评。化妆品指令的第7次修正案将导致禁止对化妆品成分进行动物试验。因此，替代策略是必要的，它允许测试化学品的广泛的物理化学性质的条件下类似于体内exposure.We最近创建了一个实验模型的细胞损伤修复更接近活体比传统的模型，通过使用分层培养角膜上皮。在眼用MFLX溶液处理和眼用LVFX溶液处理中，角膜上皮缺损修复至与阴性对照组相似的程度。此外，我们还开发了更方便的测试，使我们能够进行高通量的眼刺激测试。

The in vivo Draize eye test, which has become the international standard assay for eye irritation is often criticized for both ethical and scientific reasons. The 7th amendment of the Cosmetic directive will lead to the ban of animal testing for cosmetic ingredients. Thus, alternative strategies are necessary which allow the testing of chemicals with wide physicochemical properties under conditions similar to in vivo exposure.We recently created an experimental model of cell damage repair closer to the live body than conventional models, by using layered sheets of cultured corneal epithelium. The corneal epithelial defect was repaired in the ophthalmic MFLX solution treatment and the ophthalmic LVFX solution treatment to a degree similar to that in the negative control group. Furthermore, we have developed more convenient test that enable us to perform high through put eye irritancy test.