Development of ocular irritancy test using cultured corneal epithelial sheet

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The in vivo Draize eye test, which has become the international standard assay for eye irritation is often criticized for both ethical and scientific reasons. The 7th amendment of the Cosmetic directive will lead to the ban of animal testing for cosmetic ingredients. Thus, alternative strategies are necessary which allow the testing of chemicals with wide physicochemical properties under conditions similar to in vivo exposure.We recently created an experimental model of cell damage repair closer to the live body than conventional models, by using layered sheets of cultured corneal epithelium. The corneal epithelial defect was repaired in the ophthalmic MFLX solution treatment and the ophthalmic LVFX solution treatment to a degree similar to that in the negative control group. Furthermore, we have developed more convenient test that enable us to perform high through put eye irritancy test.