Immunology and regenerative medicine-based approach to acute lung injury

基于免疫学和再生医学的急性肺损伤方法

• 批准号: 23592681
• 负责人: FUJISHIMA Seitaro
• 金额: $ 3.33万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592681/
• 关键词: 炎症性肺疾患; 急性呼吸促迫症候群; ARDS

1) We examined the mechanisms of exaggerated bleomycin-induced acute lung injury in IL-17-deficient mice, and found that IL-12-expressing dendritic cells were increased in the knockout mice. 2) In human endothelial cells, in association with the accelerated response, gene expression of TLR and NOD families were significantly affected under hyperoxia. 3) We examined the differentiation of human embryonic stem cells to alveolar epithelial cells under air-liquid interface, using SPC and AP5 as markers. The expression of SPC was increased at 20 days, but was decreased at 40 days, whereas that of AP5 was increased with time. 4) We examined lung stem cells and progenitor cells in autopsied lungs from patients with ARDS and IPF. In ARDS, accumulation of inflammatory cells in the early phase and metaplastic epithelial cells in the late phase were observed, but Musashi-1 and SPC double positive cells were not observed. In IPF, Musashi-1 and CCSP double positive cells were also not observed.

1)研究了IL-17基因缺陷小鼠急性肺损伤的发病机制，发现基因敲除小鼠肺组织中表达IL-12的树突状细胞增多。2)在人内皮细胞中，TLR和NOD家族的基因表达在高氧条件下受到显著影响，并伴随着反应的加速。3)以SPC和AP5为标记物，研究了人胚胎干细胞在气液界面下向肺泡上皮细胞分化的情况。SPC的表达在20d时增加，40d时减少，而AP5的表达则随着时间的推移而增加。4)检测ARDS和IPF患者尸检肺组织中的肺干细胞和祖细胞。ARDS早期可见炎性细胞聚集，晚期可见化生上皮细胞，但未见Musashi-1和SPC双阳性细胞。在IPF中也未见Musashi-1和CCSP双阳性细胞。

项目成果

敗血症～病態と診断・治療～

脓毒症～病理、诊断和治疗～

• 作者: 小倉裕司;藤島清太郎;他;藤島清太郎;藤島清太郎
• 通讯作者: 藤島清太郎

ARDS:新たな定義と病態の多様性

ARDS：新定义和疾病多样性

• 发表时间: 2013
• 作者: 林田敬;藤島清太郎;Fujishima S;藤島清太郎
• 通讯作者: 藤島清太郎

急性呼吸促迫症候群:画像とバイオマーカーからの病態評価

急性呼吸窘迫综合征：图像和生物标志物的病理评估

• 发表时间: 2013
• 作者: 林田敬;藤島清太郎;Fujishima S;藤島清太郎;藤島清太郎
• 通讯作者: 藤島清太郎

急性肺損傷(ALI)、急性呼吸促迫症候群(ARDS)、最近のトピックスと基礎傷病別のエビデンス

急性肺损伤 (ALI)、急性呼吸窘迫综合征 (ARDS)、潜在损伤/疾病的最新主题和证据

• 发表时间: 2011
• 期刊: 呼吸
• 作者: Kamata H;Fujishima S;et al;藤島清太郎;藤島清太郎
• 通讯作者: 藤島清太郎

Acute Respiratory Distress Syndrome : A Case-based Approach for a Heterogenous Pathophysiological Condition

急性呼吸窘迫综合征：针对异质病理生理状况的基于病例的方法

• 发表时间: 2013
• 作者: 林田敬;藤島清太郎;Fujishima S
• 通讯作者: Fujishima S