A study of tissue trauma with hemorrhagic shock induced remote organ failure.

失血性休克引起的组织创伤导致远端器官衰竭的研究。

• 批准号: 23592686
• 负责人: OHIZUMI Akira
• 金额: $ 3.33万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592686/
• 关键词: 外傷; 遠隔臓器障害; 骨軟部組織損傷; 外傷後臓器障害; 出血性ショック; 多臓器不全; 外傷後臓器傷害

The aim of our study was to investigate the mechanism of multiple organ failure after soft tissue trauma. First, we produced an animal model of tissue trauma, and verified the validity of the model for the study of trauma. Our produced trauma model was a non-analgesic murine tissue trauma model, which was constructed by the dorsal subcutaneous implantation of minced donor syngeneic tissue-bone matrix (TBX) precisely adjusted to body weight and studyed after 21 hrs. Next, we investigated the effect of two-hit phenomenon (tissue trauma/sepsis) on gastrointestinal motility using our produced mode. Syngeneic TBX model was a highly controllable and reliable survival model of tissue injury, and two hit synergy occurred as gastrointestinal motility was only severely altered in the combined tissue trauma/sepsis model.

我们研究的目的是探讨软组织创伤后多器官衰竭的机制。首先，我们制作了组织创伤动物模型，并验证了该模型对于创伤研究的有效性。我们制作的创伤模型是非镇痛的小鼠组织创伤模型，该模型是通过将切碎的供体同源组织骨基质（TBX）精确调整至体重的背侧皮下植入并在21小时后进行研究而构建的。接下来，我们使用我们产生的模式研究了二次打击现象（组织创伤/脓毒症）对胃肠道运动的影响。同基因TBX模型是一种高度可控且可靠的组织损伤生存模型，并且在联合组织创伤/脓毒症模型中胃肠道运动仅发生严重改变，因此发生两次打击协同作用。