A study of tissue trauma with hemorrhagic shock induced remote organ failure.

本研究的目的是探讨软组织创伤后多器官功能衰竭的机制。首先，我们制作了组织创伤的动物模型，并验证了该模型对于创伤研究的有效性。我们制作的创伤模型是一种非镇痛的小鼠组织创伤模型，该模型通过将切碎的供体同基因组织-骨基质（TBX）精确地调整到体重并在21小时后研究的背部皮下植入来构建。接下来，我们研究了两次打击现象（组织创伤/脓毒症）对胃肠动力的影响，使用我们制作的模式。同基因TBX模型是一种高度可控和可靠的组织损伤存活模型，并且在联合组织创伤/脓毒症模型中，由于胃肠道动力仅严重改变，因此发生了两次打击协同作用。

The aim of our study was to investigate the mechanism of multiple organ failure after soft tissue trauma. First, we produced an animal model of tissue trauma, and verified the validity of the model for the study of trauma. Our produced trauma model was a non-analgesic murine tissue trauma model, which was constructed by the dorsal subcutaneous implantation of minced donor syngeneic tissue-bone matrix (TBX) precisely adjusted to body weight and studyed after 21 hrs. Next, we investigated the effect of two-hit phenomenon (tissue trauma/sepsis) on gastrointestinal motility using our produced mode. Syngeneic TBX model was a highly controllable and reliable survival model of tissue injury, and two hit synergy occurred as gastrointestinal motility was only severely altered in the combined tissue trauma/sepsis model.