Establishment of iPS cell lines from dental pulp cells using Sendai virus vectors

为了将iPS细胞应用于临床，正在建立无需载体整合的更安全的方法。最近，我们为此目的使用附加型质粒载体系统，并成功地建立了多个线与京都大学合作。其中一条线被提交给RIKEN BRC，并开始分发给其他研究人员。在本研究中，我们试图利用仙台病毒载体系统建立人类白细胞抗原（HLA）纯合的iPS细胞。我们还尝试了无血清培养方法，并成功建立了多株iPS细胞系。

For application of iPS cells for clinical use, safer methods without vector integration are being established. Recently, we used episomal plasmid vector system for this purpose, and succeeded to establish multiple lines in collaboration with Kyoto University. One of the lines were submitted to RIKEN BRC and started to be distributed to other researchers. In this research, we tried to use Sendai virus vector systems to establish iPS cells homozygous for human leukocyte antigens (HLAs). We also tried serum-free method for culturing the cells, and succeeded to establish multiple iPS cell lines.