Remodeling simulation of human bone structures

人体骨骼结构重塑模拟

• 批准号: 16591823
• 负责人: TEZUKA Ken-ichi
• 金额: $ 2.3万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591823/
• 关键词: Bone remodeling; Computer simulation; Finite element analysis; Reaction-diffusion system; Mandible; 骨髄炎; 再生医療; 大理石病; シミュレーションバイオロジー; 反応拡散系

Bone has a structure adapted to the mechanical environment (Wolff's law). However, the complexity of both physical and biological conditions applied to bone have prevented us from investigating the mechanism as to how bone structure can provide enough durability to multiple loading conditions. To challenge this problem, we performed a comparison of the internal structure of human mandible with those structures generated by a simulated bone remodeling method that we used successfully in a previous study to reproduce the 2-dimensional structure of the human femoral neck (Tezuka, K et al., 2005, J Bone Miner Metabol 23, 1-7). A 3-dimensional mandible bone model consisting of approximately 1.5 million elements was constructed from sequential computer tomography (CT) images of a 14-year-old female with a bone cyst. The locations and directions of muscles, and their forces were predicted from the CT images. We emulated various biting conditions, such as incisor, left/right group (LG+RG), or left/right molar (LM+RM) biting, by restricting the movement of the corresponding teeth in angled planes. Finite element method (FEM) analysis and remodeling simulation were performed one after the other, and repeated until the internal structure adapted to each loading condition was obtained. As a result, the LM+RM biting condition generated structures showing the highest degree of similarity with the real mandible. However, structures generated by other conditions also showed significant similarity with that of real mandible, suggesting that the structure of mandible has been formed to provide enough durability to various biting conditions. The precise mechanism as to how the bone cells combine the input from various conditions and generate a single structure with durability to varying mechanical environment is still unknown, however, computer-aided methodology will be a powerful tool to give us further insight into such complex problems.

骨骼具有适应机械环境的结构（沃尔夫定律）。然而，应用于骨骼的物理和生物条件的复杂性使我们无法研究骨骼结构如何为多种负载条件提供足够的耐久性的机制。为了解决这个问题，我们将人类下颌骨的内部结构与通过模拟骨重塑方法生成的结构进行了比较，我们在之前的研究中成功地使用了该方法来重现人类股骨颈的二维结构（Tezuka, K et al., 2005, J Bone Miner Metabol 23, 1-7）。根据一名患有骨囊肿的 14 岁女性的连续计算机断层扫描 (CT) 图像构建了一个由大约 150 万个元素组成的 3 维下颌骨模型。根据 CT 图像预测肌肉的位置、方向及其力量。我们通过限制相应牙齿在成角度平面中的运动来模拟各种咬合条件，例如门牙、左/右群 (LG+RG) 或左/右磨牙 (LM+RM) 咬合。有限元法（FEM）分析和重塑模拟相继进行，并重复进行，直到获得适合每种载荷条件的内部结构。结果，LM+RM咬合条件生成的结构与真实下颌骨的相似度最高。然而，其他条件下生成的结构也显示出与真实下颌骨的显着相似性，这表明下颌骨的结构已经形成，可以为各种咬合条件提供足够的耐用性。骨细胞如何结合各种条件的输入并生成对不同机械环境具有耐久性的单一结构的精确机制仍然未知，但是，计算机辅助方法将成为一个强大的工具，让我们进一步深入了解此类复杂问题。

项目成果

Computer-simulated bone architecture in a simple bone-remodeling model based on a reaction-diffusion system.

基于反应扩散系统的简单骨重塑模型中的计算机模拟骨结构。

• 发表时间: 2005
• 期刊: J. Bone Miner. Metabol. 23
• 作者: Terao;Y.;Okamoto;et al.;Masuda Y;Ken-ichi Tezuka
• 通讯作者: Ken-ichi Tezuka

培養細胞実験ハンドブック

培养细胞实验手册

• 发表时间: 2004
• 作者: Yoshida;A.;手塚 建一
• 通讯作者: 手塚 建一

Identification of genes expressed at the condensation stage of a chondrogenic cell line, ATDC5, by the SST-REX method.

通过 SST-REX 方法鉴定软骨形成细胞系 ATDC5 浓缩阶段表达的基因。

• 发表时间: 2006
• 期刊: J. Bone Miner. Metabol. 24
• 作者: Cheng J;Maruyama S;Suzuki M;Fujita H;Takagi R;Saku T;Ken-ichi Tezuka;Atsushi Noguchi
• 通讯作者: Atsushi Noguchi

Computer simulation of human mandible bone structure by iBone, a novel reaction-diffusion bone remodeling model.

iBone 是一种新型反应扩散骨重建模型，对人体下颌骨结构进行计算机模拟。

• 发表时间: 2006
• 期刊: Key Engineering Materials 306-308
• 作者: Cheng J;Maruyama S;Suzuki M;Fujita H;Takagi R;Saku T;Ken-ichi Tezuka
• 通讯作者: Ken-ichi Tezuka

Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse a2(XI) collagen promoter.

Cre 重组酶在由小鼠 a2(XI) 胶原蛋白启动子驱动的小鼠发育软骨细胞中的表达。

• 发表时间: 2005
• 期刊: J. Bone Miner. Metabol. 23
• 作者: Okamoto;S.;Kawabata;S.;Terao;Y.;Fujitaka;H.;Okuno;Y.;Hamada,S.;Ryoji Fujimaki
• 通讯作者: Ryoji Fujimaki