Radioprotective assay on bone metabolism with Japanese Medaka (Oryzias latipes)

日本青鳉（Oryzias latipes）骨代谢的辐射防护测定

• 批准号: 23592765
• 负责人: SAWAJIRI Masahiko
• 金额: $ 3.33万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592765/
• 关键词: メダカ(Oryzias); 放射線照射; 破骨細胞誘導; シグナル伝達物質; 骨浸潤性腫瘍; がん放射線治療; 実験腫瘍学; がん治療; 咽頭歯骨; 破骨細胞

We investigated bone metabolism signaling protein biochemically such as osteoclast-induced cytokine using the pharyngeal bones of medaka (Oryzias) after irradiation. Periostin, which frequency expressed osteoblast, was found in bone invasive tumors and also could significantly affect on bone metabolism. Moreover, periostin was expressed on the calcification and the maturation of osteoblast. However, the effects of periostin on bone metabolism are still not clear about carbon ion irradiation enhanced the expression of periostin on osteoblast cells. The result of this study showed that carbon ion irradiation can increase periostin on osteoblast cell. Furthermore, the irradiated osteoblast cell also express high representation of calcification factor and bone maturation factor. Carbon ion radiotherapy might promote the prevention of bone absorption and the pathological fracture after exposure irradiation.

本研究以辐射后青鳉的咽骨为材料，对破骨细胞诱导的细胞因子等骨代谢信号蛋白进行了生化研究。Periostin在骨侵袭性肿瘤中大量表达，对骨代谢也有重要影响。骨膜蛋白在成骨细胞的钙化和成熟过程中有表达。然而，骨膜蛋白对骨代谢的影响尚不清楚，碳离子照射是否增强了成骨细胞上骨膜蛋白的表达。本研究结果表明，碳离子辐照可使成骨细胞膜蛋白含量增加。此外，照射后的成骨细胞还表达高表达的钙化因子和骨成熟因子。碳离子放射治疗可促进骨吸收的预防和照射后病理性骨折的发生。

项目成果

The extracts of Japanese willow tree species are effective forapoptotic desperation or differentiation of acute myeloid leukemia cells.

• DOI: 10.4103/0973-1296.131023
• 发表时间: 2014-04
• 期刊: Pharmacognosy magazine
• 影响因子: 0.7
• 作者: Fujita K;Nomura Y;Sawajiri M;Mohapatra PK;El-Shemy HA;Nguyen NT;Hosokawa M;Miyashita K;Maeda T;Saneoka H;Fujita S;Fujita T
• 通讯作者: Fujita T

Irradiatin effect on osteoclastgenesis stimulated

刺激破骨细胞生成的辐照作用

• DOI: 10.1097/hp.0b013e3182118333
• 发表时间: 2012
• 期刊: Health Physics
• 影响因子: 2.2
• 作者: M Tsumura;Y Shibukawa;T Muramatsu;U Sobhan;M Sato;H Ichikawa;M Tazaki;Masahiko Sawajiri
• 通讯作者: Masahiko Sawajiri

重粒子線照射の乳がん細胞の転移におよぼす影響

重离子束照射对乳腺癌细胞转移的影响

• 发表时间: 2013
• 作者: Takeuchi H;Hirano T;Whitmore SE;Morisaki I;Amano A;Lamont RJ;佐藤正樹,津村麻記,Ubaidus Sobhan,市川秀樹,村松敬,田崎雅和,澁川義幸;澤尻 昌彦
• 通讯作者: 澤尻 昌彦

重粒子線の骨代謝におよぼす影響

重离子束对骨代谢的影响

• 发表时间: 2014
• 作者: 澤尻昌彦;野村雄二,寺東宏明,丸山耕一,谷本啓二
• 通讯作者: 野村雄二,寺東宏明,丸山耕一,谷本啓二

乳がん細胞の破骨細胞誘導における放射線の影響

辐射对乳腺癌细胞破骨细胞诱导的影响

• 发表时间: 2013
• 作者: 澤尻昌彦;スリマウォング・プリヤポーン,野村雄二,寺東宏明,丸山耕一,谷本啓二
• 通讯作者: スリマウォング・プリヤポーン,野村雄二,寺東宏明,丸山耕一,谷本啓二