Study of inflammatory and anti-inflammatory mechanisms by endothelial cells in persistent periapical periodontitis

持续性根尖周炎内皮细胞炎症及抗炎机制研究

• 批准号: 23592811
• 负责人: TAKEICHI Osamu
• 金额: $ 3.24万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592811/
• 关键词: 歯根肉芽種; midkine; 誘導型一酸化窒素合成酵素; CX3CL1; 血管内皮細胞; 遺伝子発現; Porphyromonas gingivalis; LPS; 根尖性歯周炎; 炎症性細胞; 慢性根尖性歯周炎; 臍帯静脈血由来血管内皮細胞; real-time PCR; 難治性根尖性歯周炎; CD105; 蛍光二重免疫染色法; real time PCR法

The aim of this study was to determine the function of midkine and iNOS inhibitor to endothelial cells in periapical granulomas. Midkine, iNOS and CXCL1 protein and mRNA expression in 69 periapical granulomas were detected, although healthy gingival tissues did not show those expressions. LPS was extracted from Porphyromonas gingivalis, which is known as potent periodontopathic bacteria. Then, human umbilical vein endothelial cells (HUVEC) were stimulated with the LPS. Stimulated HUVEC showed iNOS, midkine and CXCL1 protein and mRNA expression, but not after the application of iNOS inhibitor. The data are consistent with a hypothesis suggesting that endothelial cells in periapical granulomas express both iNOS and midkine, and these molecules may contribute tissue remodeling.

本研究的目的是确定中期因子和诱导型一氧化氮合酶抑制剂对根尖周肉芽肿内皮细胞的作用。在69例根尖周肉芽肿组织中检测到中期因子、iNOS和CXCL1蛋白和mRNA的表达，而健康牙龈组织未显示这些表达。LPS是从牙龈卟啉单胞菌中提取的，牙龈卟啉单胞菌是已知的强效牙周病细菌。然后，用LPS刺激人脐静脉内皮细胞（HUVEC）。刺激的HUVEC显示iNOS、中期因子和CXCL 1蛋白和mRNA的表达，但应用iNOS抑制剂后不表达。这些数据与提示根尖周肉芽肿中内皮细胞同时表达iNOS和中期因子的假设一致，这些分子可能有助于组织重塑。

项目成果

IL-6 and soluble IL-6 receptor stimulate the production of MMPs and their inhibitors via JAK-STAT and ERK-MAPK signaling in human chondrocytes

IL-6 和可溶性 IL-6 受体通过人软骨细胞中的 JAK-STAT 和 ERK-MAPK 信号传导刺激 MMP 及其抑制剂的产生

• 发表时间: 2012
• 期刊: Cell Biology International
• 影响因子: 3.9
• 作者: Aida Y;Honda K;Tanigawa S;Nakayama G;Matsumura H;Suzuki N;Shimizu O;Takeichi O;Makimura M;Maeno M.
• 通讯作者: Maeno M.

歯根肉芽種のおけるmidkineとchemokine遺伝子発現

根粒化物种中中期因子和趋化因子基因的表达

• 发表时间: 2012
• 作者: 羽鳥啓介;武市収;牧野公亮;小林寛;兼坂絵理奈;鶴町保;小木曾文内
• 通讯作者: 小木曾文内

The periodontal pathogen Porphyromonas gingivalis induces the Epstein-Barr virus lytic switch transactivator ZEBRA by histone modification

• DOI: 10.1016/j.biochi.2011.12.001
• 发表时间: 2012-03-01
• 期刊: BIOCHIMIE
• 影响因子: 3.9
• 作者: Imai, Kenichi;Inoue, Harumi;Ochiai, Kuniyasu
• 通讯作者: Ochiai, Kuniyasu

歯根肉芽種におけるEpstein-Barr virusの検出

根肉芽肿中 Epstein-Barr 病毒的检测

• 发表时间: 2013
• 作者: 牧野公亮;武市収;羽鳥啓介;勝呂尚;小木曽文内;今井健一;落合邦康
• 通讯作者: 落合邦康

難治性根尖性歯周炎におけるmidkineとiNOS発現

难治性根尖周炎中中期因子和 iNOS 的表达

• 发表时间: 2013
• 作者: 武市 収;牧野公亮;羽鳥啓介;小木曾文内
• 通讯作者: 小木曾文内