An investigation of the relationship between occlusion, mastication and mental activity using in vivo patch clamp recording method

在这个项目中，我们试图为体内膜片钳技术提供一种微移液管。不幸的是，很少有探头可以形成高电阻密封（千兆欧密封）。另一种装置也需要到达活体标本的目标神经元。然而，这些微移液器允许我们进行细胞外记录。细胞外记录了兔丘脑腹侧后内侧核对口面区机械刺激反应的神经元活动。90%的记录神经元对与咬合和咀嚼相关的机械刺激有反应，也与心理活动有关。这些结果表明，咬合和咀嚼过程中来自口面区的感觉信息对意识水平有影响。

In this project we try to provide a micropipette for the in vivo patch clamp technique. Unfortunately, few probes were obtained to allow forming the high resistance seal (giga-ohm seal). The other device was also needed to reach the objective neurons of the in vivo specimen. These micropipettes, however, allowed us performing extracellular recording. Neuronal activities responding mechanical stimulation to the oro-facial region were extracellularly recorded from the ventral posteromedial nucleus of the rabbit thalamus. Ninety percent of recorded neurons responded to mechanical stimuli associated with occluding and chewing and also related to the mental activity. These results suggested that sensory information from oro-facial region during occlusion and mastication influences on the level of consciousness.