The study of cementum regeneration in epithelial-mesenchymal interaction

将牙周膜成纤维细胞与马拉色上皮细胞共培养。Malassez上皮细胞与牙周膜成纤维细胞流的相互作用诱导了Malassez上皮细胞中釉原蛋白、成釉蛋白、MMP-20和KLK 4的表达。提示上皮-间充质相互作用增强了Malassez上皮细胞的细胞更新。这些结果表明上皮-间充质相互作用增强了Malassez上皮细胞的细胞更新。间充质相互作用可诱导Malassez剩余部分上皮细胞中的细胞更新和细胞牙骨质形成。

Both the epithelial cells of Malassez and periodontal ligament fibroblasts were co-cultured in the same dish. The interactions between the epithelial cells of Malassez and periodontal ligament fibroblastscurrent induceed the expression of amelogenin, ameloblastin, MMP-20 and KLK 4 in the epithelial cells of Malassez.We also showed that the epithelial cells of Malassez which incorporated BrdU expressed Bax strongly in co-cultured cells, suggesting that the epithelial-mesenchymal interactions enhance cell turnover in the epithelial cells of Malassez.These results indicate that epithelial-mesenchymal interactions may induce cell turnover and cellular cementum formationin in the epithelial cells of the rests of Malassez.