The application of functional elucidation in epithelial-mesenchymal interaction to the practical treatment in chronic periodontitis

上皮间质相互作用的功能阐明在慢性牙周炎实际治疗中的应用

• 批准号: 19592167
• 负责人: SHIMONISHI Mitsuru
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592167/
• 关键词: 免疫; 感染; 炎症; マラッセの上皮遺残; MMP-2; 細胞間相互作用; 免疫・感染・炎症

The tissues were sampled from the root of human extracted teeth and produced outgrowths containing both epithelial cells and fibroblasts from human periodontal ligaments (HPDL). Type IV collagen were found at the interface between cells of the epithelial rests of Malassez (ERM cells) and HPDL fibroblasts. MMP-2 can degrade type IV collagen. The current study was undertaken to examine the expression of MMP-2 at the interface. These findings indicate that the ERM cells stimulate the production of MMP-2 in HPDL fibroblasts. Up-regulated MMP-2 activated by MMP-14 in ERM cells could degrade matrix molecules, such as Type IV collagen in the basal membrane between ERM cells and HPDL fibroblasts. We believe that these results are applied to the practical treatment in chronic periodontitis.

这些组织取自人类拔除的牙齿的根部，并产生含有人牙周韧带(HPDL)上皮细胞和成纤维细胞的突起。在Malassez上皮细胞(ERM细胞)与HPDL成纤维细胞的交界处可见IV型胶原。基质金属蛋白酶-2能降解IV型胶原。本研究旨在检测基质金属蛋白酶-2在细胞界面的表达。这些结果表明，ERM细胞刺激HPDL成纤维细胞产生基质金属蛋白酶-2。在ERM细胞中，由MMP14激活的MMP2表达上调可降解基质分子，如位于ERM细胞和HPDL成纤维细胞之间的基底膜中的IV型胶原。我们相信，这些结果将应用于慢性牙周炎的实际治疗。

项目成果

Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament

人牙周膜上皮细胞和成纤维细胞界面处 MMP-2 的诱导

• 发表时间: 2010
• 期刊: J Periodont Res
• 作者: Shimonishi M;Takahashi I;Terao F;Komatsu M;Kikuchi M
• 通讯作者: Kikuchi M

Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament.

在人牙周膜上皮细胞和成纤维细胞之间的界面处诱导 MMP-2。

• 发表时间: 2010
• 期刊: J Periodont Res (in press)
• 作者: Shimonishi M;Takahashi I;Terao F;Komatsu M;Kikuchi M
• 通讯作者: Kikuchi M