The role of miRNA on the mechanism of metastasis induced by the SDF-1/CXCR4 system in oral cancer

miRNA在SDF-1/CXCR4系统诱导口腔癌转移机制中的作用

• 批准号: 23592964
• 负责人: UCHIDA Daisuke
• 金额: $ 3.33万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592964/
• 关键词: CXCR4; miR-518c-5p; 口腔癌; miRNA; 転移

We examined the microRNA downstream of CXCR4 system that is one of a metastatic system in oral cancer. We identified the upregulation of miR-518c-5p, which is reported as an oncomiR in bladder cancer. A miR-518c-5p inhibitor significantly inhibited the CXCR4 system-dependent migration of oral cancer cells. The growth and migration of B88-518c cells, transfected by the miR-518c expression vector, were significantly enhanced. Furthermore, tumor volume, lymph nodes metastasis, and lung metastasis were significantly increased in the nude mice inoculated with B88-518c cells. These results indicated that miR-518c-5p regulates the metastases of oral cancer as a downstream target of CXCR4 system.

我们检测了CXCR 4系统下游的microRNA，CXCR 4系统是口腔癌转移系统之一。我们鉴定了miR-518 c-5 p的上调，其被报道为膀胱癌中的oncomiR。 miR-518 c-5 p抑制剂显著抑制口腔癌细胞的CXCR 4系统依赖性迁移。转染miR-518 c表达载体的B88- 518 c细胞的生长和迁移显著增强。 B88- 518 c细胞接种裸鼠后，肿瘤体积、淋巴结转移和肺转移明显增加。 这些结果表明，miR-518 c-5 p作为CXCR 4系统的下游靶点调节口腔癌的转移。

项目成果

Stromal-cell derived factor(SDF)-1/CXCR4システムにより誘導される口腔癌の転移関連マイクロRNAの検索

寻找与基质细胞衍生因子（SDF）-1/CXCR4系统诱导的口腔癌转移相关的microRNA

• 发表时间: 2013
• 作者: Okui G;Tobiume K;Rizqiawan A;Yamamoto K;Shigeishi H;Ono S;Higashikawa K;Kamata N;岸本晃治7名;Kawakami T;木内誠,内田大亮,栗林伸行,玉谷哲也,永井宏和,宮本洋二
• 通讯作者: 木内誠,内田大亮,栗林伸行,玉谷哲也,永井宏和,宮本洋二

Expression and function of CXCR4 in human salivary gland cancers

• DOI: 10.1007/s10585-012-9518-9
• 发表时间: 2013-02-01
• 期刊: CLINICAL & EXPERIMENTAL METASTASIS
• 影响因子: 4
• 作者: Uchida, Daisuke;Kuribayashi, Nobuyuki;Miyamoto, Youji
• 通讯作者: Miyamoto, Youji