Basic analysis for evaluating the possibility of phytochemicals as a therapeutic and prophylactic agent for mood disorders

白藜芦醇因其具有抗氧化、抗凋亡和抗炎作用而受到广泛关注。我们以前报道过白藜芦醇通过抑制培养的牛肾上腺髓质细胞中的离子内流来抑制乙酰胆碱诱导的儿茶酚胺的分泌和合成。在这项研究中，我们研究了白藜芦醇对去甲肾上腺素（NA）转运蛋白的影响，这也是已知的抗抑郁药的细胞靶点。低浓度白藜芦醇促进SK-N-SH细胞摄取NA，高浓度白藜芦醇抑制SK-N-SH细胞摄取NA。动力学分析表明，白藜芦醇可降低SK-N-SH细胞NA转运的Vmax，但对Km无明显影响。Scatchard分析nisoxetine与细胞膜的结合表明，白藜芦醇不影响Bmax和Kd。本研究结果表明，白藜芦醇在调节神经元NA转运中发挥着双相作用。

Resveratrol has attracted wide attention because of its antioxidant, anti-apoptotic and anti-inflammatory effects. We have previously reported that resveratrol inhibits acetylcholine-induced catecholamine secretion and synthesis through suppressing ion influx in cultured bovine adrenal medullary cells. In this study, we examined the effect of resveratrol on noradrenaline (NA) transporter, which is also known to be the cellular targets of antidepressants. Resveratrol stimulated NA uptake by SK-N-SH cells at low concentration, it was inhibited NA uptake at high concentration against this. Kinetics analysis of the effect of resveratrol on NA transport in SK-N-SH cells indicated that they decreased the Vmax of NA transport with little or no change in Km. Scatchard analysis of nisoxetine binding to plasma membranes showed that resveratrol did not affect the Bmax and the Kd.The present findings suggest that resveratrol plays a biphasic role in the regulation of neuronal NA transport.