Quality control of iPS cells by studying differentiation resistance.

根据四个重编程因子的表达水平，开发了一个标签系统来对诱导多能干细胞(IPS)进行分类。有效率(Oct3/4-高，Sox2-低，K1F4-高，c-Myc-高)。为了研究其分子基础，进行了基因芯片分析。途径分析表明，在高效条件下，G蛋白偶联受体(GPCR)途径显著上调，趋化因子C-C基序配体2促进体细胞重编程。此外，遗传修饰物Whsc1l1(变体1)也提高了体细胞重编程的效率。

A tagging system was developed to sort induced pluripotent stem (iPS) cells according to the expression levels of each of the four reprogramming factors. Using this system, the effective ratio (Oct3/4-high, Sox2-low, Klf4-high, c-Myc-high) was. To investigate the molecular basis, microarray analysis was performed. Pathway analysis revealed that the G protein-coupled receptor (GPCR) pathway was up-regulated significantly under the high efficiency condition and treatment with the chemokine, C-C motif ligand 2, a member of the GPCR family, enhanced somatic cell reprogramming. Furthermore, the genetic modifier, Whsc1l1 (variant 1), also improved the efficiency of somatic cell reprogramming.