Generation of a transgenic mouse which can be used for the in vivo detection of CREB phosphorylatio

在2009财年，我们通过调整麻醉时间和麻醉量，成功开发了麻醉小鼠的CREB磷酸化检测系统。在这一年，我们成功地观察了非麻醉小鼠的CREB磷酸化。我们证实氟西汀急性给药可上调小鼠脑光强度，表明CREB磷酸化。我们也通过westernblotting证实氟西汀治疗后CREB磷酸化上调。

In 23rdfiscal year, we succeeded in developing the CREB phosphorylation detecting system using anesthesized mouse by adjasting the timing and amount of anesthesia. In this year, we succeeded in observing CREB phosphorylation in non-anesthesized mouse. We confirmed acute adminidtration of Fluoxetine up-regulated the light intensity from mouse brain which indicated the CREB phosphorylation. And we also confirmed the up-regulation of CREB phosphorylation after Fluoxetine treatment by westernblotting.