Production of new design mice by accumulation of mutations

为了建立新的模型来研究体内控制数量性状的突变网络，我们建立了几个突变积累（MA）系，并对突变小鼠进行了长期育种。在数量性状（体重、体长、尾长）方面，我们观察到不同MA品系之间和品系内部的差异。生化分析显示，MA系包括肝脏或肾脏功能障碍或动脉硬化的小鼠。NGS分析显示，MA系有大量的新生突变。我们利用MA系的方法将为理解哺乳动物模型的数量遗传学提供一种新的途径。

To create new model to investigate mutational network controlling quantitative traits in vivo, we established several mutation accumulation (MA) lines with long term breeding of mutator mice. We observed differences in quantitative traits (weight, body length, tail length) between and within each of MA line. Biochemical analysis revealed MA lines include mice having dysfunctions of liver or kidney or arteriosclerosis. NGS analysis showed abundant de novo mutation in MA lines. Our approach using the MA lines would provide a new way to understand quantitative genetics in mammalian model.