Optical control of cell activity by introduction of photo-switching compound

通过引入光开关化合物对细胞活动进行光学控制

• 批准号: 23650259
• 负责人: MAGOME Nobuyuki
• 金额: $ 2.16万
• 依托单位: Dokkyo Medical University (2012-2013)Kyoto University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650259/
• 关键词: 光スイッチング; 心筋細胞; 興奮性; 光制御; ナノ粒子; 細胞導入; アゾ化合物; HDL; 金ナノロッド; 紫外―可視

In this research subject, we made nano-sized complex, consisting of lipid, protein, and photo-switching molecules, and then the complex were introduced into cultivated cardiomyocyte in order to realize optical control of excitation of the cells. We found that the complexes were easily taken into the cells, and also were disassembled within the cell. As a result, the photo-switching molecules diffuse inside the cell, and then interact with cell membrane. In the research process, it was observed that structure of cell membrane was reversibly modified by the photo-sensitive substance. According to this result, it is assumed that the change of structure causes to increase membrane permeability of ions and other substances.

本课题通过制备脂质、蛋白质和光开关分子组成的纳米复合物，并将其导入培养的心肌细胞中，实现对细胞激发的光控制。我们发现，复合物很容易被带入细胞，也在细胞内被分解。因此，光开关分子在细胞内扩散，然后与细胞膜相互作用。在研究过程中，观察到光敏物质对细胞膜结构的可逆修饰。根据这一结果，可以认为结构的变化导致离子和其他物质的膜渗透性增加。

项目成果

Digital photo-control of the network of live excitable cells

活体可兴奋细胞网络的数字光控制

• DOI: 10.1134/s0021364011180056
• 发表时间: 2011
• 期刊: J.Exp.Theor.Phys.Lett.
• 作者: I.S.Erofeev;N.Magome;K.Agladze
• 通讯作者: K.Agladze

Photo-control of excitation waves in cardiomyocyte tissue culture.

心肌细胞组织培养中激发波的光控制。

• 发表时间: 2011
• 期刊: Tissue Engineering. Part A
• 作者: N. Magome;G. Kanaporis;N. Moisan;Koichiro Tanaka;K. Agladze
• 通讯作者: K. Agladze

Curvature-Dependent Excitation Propagation in Cultured Cardiac Tissue.

• DOI: 10.1134/s0021364011230044
• 发表时间: 2012-02
• 期刊: JETP letters
• 影响因子: 1.3
• 作者: Kadota S;Kay MW;Magome N;Agladze K
• 通讯作者: Agladze K

Electronspun nanofibers as a tool for architecture control in engineered cardiac tissue

电纺纳米纤维作为工程心脏组织结构控制的工具

• 发表时间: 2011
• 期刊: Biomaterials
• 影响因子: 14
• 作者: Y. Orlova;N. Magome;L. Liu;Y. Chen;and K. Agladze
• 通讯作者: and K. Agladze

Multi-electrode monitoring of guided excitation in patterned cardiomyocytes

• DOI: 10.1016/j.mee.2012.11.018
• 发表时间: 2013-11
• 期刊: Microelectronic Engineering
• 影响因子: 2.3
• 作者: Li Wang;Li Liu;Xin Li;N. Magome;K. Agladze;Yong Chen