Raman spectrum analysis of cell differentiation dynamics

人乳腺癌细胞系MCF-7在向培养基中加入调蛋白后分化成哺乳动物巨噬细胞样细胞。在这项研究中，我们跟踪细胞内部状态的变化，通过拉曼光谱测量，这是一个有效的方法来检测多维的细胞内动态在单细胞与最小的破坏。直到分化过程的12天的拉曼动力学分析揭示了主要细胞内组分的假圆形轨迹。

A human breast cancer cell line, MCF-7 differentiates into mammalian grand-like cells upon addition of heregulin to the culture medium. In this study, we traced changes of cellular internal states by means of Raman spectrum measurement, which is a potent method to detect multi-dimensional intracellular dynamics in single cells with minimal disruption. Analysis of the Raman dynamics up to 12 days though the differentiation process has revealed pseud-circular trajectory of the major intracellular components.