Domain structure of the plasma membrane : interaction between membrane skeleton and membrane receptors

质膜的域结构：膜骨架和膜受体之间的相互作用

• 批准号: 04833003
• 负责人: SAKO Yasushi
• 金额: $ 1.28万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04833003/
• 关键词: plasma membrane; membrane proteins; transiferrin receptor; coated pitt; adoptor complex; single particle tracking; optical twtezems; cytoskoleton; 一粒子追路法; 膜ドメイン; 側方拡散; 膜骨格; レーザー光トラップ

Movements of transferrin receptor molecules in the plasma membrane of cultured normal rat kidney fibroblastic cells were investigated by video-enhanced contrast optical microscopy with nanometer-level special precision and video-rate temporal resolution by lebeling the receptor with the ligand-coated 40-nm colloidal gold particles. Most of the movement trajectories are of the confined diffusion type within domains of -0.25mum^2. Movement within the domains is random with a diffusion coefficient -10^<-9> cm^2/s, which is consistent with that expected for free Brownian diffusion of proteins in the plasma membrane. The receptor molecules move from one domain to one of the adjacent domains at an average frequency of 0.034s^<-1>, indicating that the plasma membrane is compartmentalized for diffusion of membrane receptors and that long-range diffusion is the result of successive intercompartmental jumps. Partial destruction of the cytoskeleton suggest that the boundaries between compartments are made of dynamically fluctuating membrane skeletons.

用40 nm胶体金标记转铁蛋白受体，用视频增强对比光学显微镜观察了转铁蛋白受体分子在培养的正常大鼠肾成纤维细胞质膜上的运动。在-0.25 mum ^[2]的区域内，大部分运动轨迹都属于受限扩散型。区域内的运动是随机的，扩散系数为-10 ^<-9>cm^2/s，这与蛋白质在质膜中的自由布朗扩散的预期一致。受体分子以0.034s-1的平均频率从一个结构域移动到相邻结构域之一<-1>，表明质膜被划分为用于膜受体的扩散，并且长程扩散是连续的室间跳跃的结果。细胞骨架的部分破坏表明，隔间之间的边界是由动态波动的膜骨架。

项目成果

Kusumi A.et al.: "Confined lateral diffusion of membrane receptors as observed by single particle tracking(nanovid microscopy):Effects of calcium-induced differentiation of epithelial cells." Biophysical Journal. 65. 2021-2040 (1993)

Kusumi A.等人：“通过单粒子追踪（纳米维德显微镜）观察到的膜受体的受限横向扩散：钙诱导的上皮细胞分化的影响。”

Kusumi, A., Sako, Y., and Yamamoto, M.: "Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells." Biophys.J.65. 2021-2040 (1993)

Kusumi, A.、Sako, Y. 和 Yamamoto, M.：“通过单粒子追踪（纳米维德显微镜）研究膜受体的有限横向扩散。钙诱导的培养上皮细胞分化的影响。”

Oida, T., Sako, Y., and Kusumi, A.: "Fluorescence lifetime imaging microscopy (flimscopy). Methodology development and application to studies of endosome fusion in single cells." Biophys.J.64. 676-685 (1993)

Oida, T.、Sako, Y. 和 Kusumi, A.：“荧光寿命成像显微镜（薄镜）。方法开发及其在单细胞内体融合研究中的应用。”

Oida,T.et al.: "Fluorescence lifotime imaging microscopy(flimscopy).Methodology development and application to studies of endosome fusion in single cells." Biophysical Journal. (1993)

Oida,T.et al.：“荧光实时成像显微镜（flimscopy）。单细胞内体融合研究的方法学开发和应用。”

Sako,Y.et al.: "Compartmeutalized structure of the plasmamembrane for receptor movements as revealed by a nanoweter-level motion analysis" The Jorrnal of Cell Biology. 125. 1251-1264 (1994)

Sako,Y.等人：“通过纳米级运动分析揭示了受体运动的质膜的分隔结构”《细胞生物学杂志》。