Development ofgenetically modified hepatic cells with induced high liver functions for bioartificial liver systems

在这里，我们试图通过过表达富含肝脏的转录因子(LETF)来创造具有增强肝功能的基因工程肝细胞，LETF与肝脏特异基因的转录和肝脏分化相关。用逆转录病毒载体转导小鼠肝癌细胞Hepa1-6，在逆转录病毒载体中引入LETF基因的可诱导表达盒。建立了可诱导表达高肝功能的细胞克隆。这种方法可能在构建用于生物人工肝系统的细胞方面很有前途。

Here, we attempted to create genetically engineeredhepatic cells with enhanced liver functions by overexpression of liver-enrichedtranscription factors (LETFs), which are associated with the transcription of liver-specific genes and hepatic differentiation. Mouse hepatoma Hepa1-6 cells were transduced with retroviral vectors, in which inducible expression cassettes for the LETF genes were introduced. Cell clones with inducible expression of high liver functions were established. This approach may be promising for the construction of cells for use in bioartificial liver systems.