Identification of possible biomarkers for the efficient physiotherapy after the CNS injury.

为了量化中枢神经系统损伤后物理治疗的疗效，我们通过结合运动神经元选择性核/细胞分类和下一代测序仪的转录组分析来评估康复/停用前后基因表达的变化。胸中水平脊髓全横断后，于伤后3个月用激光显微解剖采集腰椎膨大平面的运动神经元，并进行基因表达分析。这种方法将有助于识别可能的生物标志物，以建立有效的康复计划。

To quantify the efficacy of physiotherapy after the central nervous system injury, we evaluate gene expression changes between before and after rehabilitation/disuse by combining selective nuclear/cellular sorting of motor neurons and transcriptome analysis using next-generation sequencer. After the complete transection of spinal cord at mid-thoracic level, we collected motor neurons located in the level of lumbar intumescence with laser micro dissection at three months after injury, and performed gene expression analysis. This method will contribute to identify the possible biomarkers for establishment of efficient rehabilitation programs.