Bioimaging technique to observe skeletal muscle metabolites in vivo

生物成像技术观察体内骨骼肌代谢物

• 批准号: 23650403
• 负责人: KANO Yutaka
• 金额: $ 2.41万
• 依托单位: The University of Electro-Communications
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650403/
• 关键词: バイオイメージング; 骨格筋; ホメオスタシス; 筋収縮; ミトコンドリア; 細胞内pH

Using an in vivo bioimaging model and microinjection technique, we tested homeostasis of intracellular when low pH solution was loaded in single fiber by microinjection. The intact spinotrapezius muscle of adult male Wistar rats was exteriorized and loaded with the fluorescent probe BCECF-AM. Muscle was loaded low pH solution by microinjection. This model offers a unique opportunity to investigate the consequences of experimental manipulations of pH on muscle buffering dynamics under close-to-physiological conditions

利用体内生物成像模型和显微注射技术，测试了在单个纤维中注入低pH溶液时的细胞内动态平衡。取成年雄性Wistar大鼠完整的斜方肌，外置，用荧光探针BCECF-AM标记。肌肉内注入低pH溶液。这个模型提供了一个独特的机会来研究在接近生理的条件下，实验操作的pH对肌肉缓冲动力学的影响。

项目成果

伸張性筋収縮後の細胞内ナトリウムイオンの可視化

拉伸肌肉收缩后细胞内钠离子的可视化

• 发表时间: 2011
• 作者: Kondo;Y. and McNamee;M.J;木村卓磨,稲垣薫克,田中嘉法,狩野豊
• 通讯作者: 木村卓磨,稲垣薫克,田中嘉法,狩野豊

In vivo measurement of intercellular pH and contractile performance during repetitive stimulation in rat skeletal muscle

大鼠骨骼肌重复刺激期间细胞间 pH 值和收缩性能的体内测量

• 发表时间: 2012
• 作者: Yoshinori Tanaka;Tadakatsu Inagaki;Takuma Kimura;David C Poole;Yutaka Kano
• 通讯作者: Yutaka Kano